Staphylococcus aureus infections acquired in hospitals now cause more deaths per annum in the US than does HIV/AIDS. Perhaps even more alarming is the rise in community associated methicillin-resistant S. aureus (CA-MRSA) infections, which have spread out of hospital settings and are infecting otherwise healthy individuals. The mechanism of enhanced pathogenesis in CA-MRSA remains unclear, but ...

Overview Purpose: To develop fast and sensitive approaches for the analysis of underivatized free amino acids and peptides using the latest UHPLC column technology and charged aerosol detection. Methods: Presented here are two distinct methods using the same UHPLC analytical column. The first method uses large amounts of ion-pairing agents to resolve 18 free amino acids. The second method uses ...

Over the past decade the application of non-targeted high-throughput metabolomic analysis within the plant sciences has gained ever increasing interest and has truly established itself as a valuable tool for plant functional genomics and studies of plant biochemical composition. Whilst proton nuclear magnetic resonance ((1)H-NMR) spectroscopy is particularly appropriate for the analysis of bulk...

A well-characterized and fully validated ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometric (UHPLC-ESI-MS/MS) method was developed to reliably analyze combination of perindopril arginine and amlodipine besylate in bulk and tablet formulations. The chromatographic separation was achieved on a Waters ACQUITY UPLC® BEH C18 column with 1.7 μm particle pac...

Rhizoma Smilacis glabrae, a traditional Chinese medicine (TCM) as well as a functional food, has been commonly used for detoxification treatments, relieving dampness and as a diuretic. In order to quickly define the chemical profiles and control the quality of Smilacis glabrae, ultra high performance liquid chromatography coupled with electrospray ionization hybrid linear trap quadrupole orbitr...

A rapid, ultra high-performance liquid chromatographic (UHPLC) method has been developed and validated for simultaneous identification and analysis of the isoflavones genistein, daidzein, glycitin, puerarin, and biochanin A, and the flavonoids (±)-catechin, (-)-epicatechin, rutin, hesperidin, neohesperidin, quercitrin, and hesperetin in human urine. Urine samples were incubated with β-glucuroni...

We report the first rapid ultra-high performance liquid chromatographic (UHPLC) enantiomeric reversed-phase separation of rasagiline mesylate and its tartrate salts using a Chiralpak® AGP column (50 mm × 2.1 mm, 5 μm) as a stationary phase. This method was developed as an alternative to the usage of previously reported normal-phase chiral LC columns for isomer separation. Our method is based on...

Cleaning validation (CV) is a Good Manufacturing Practices (GMP) requirement in manufacturing of drug substances and drug products. Liquid Chromatography with UV detection is a common choice for cleaning verifi cation studies for most drugs at sensitivity levels of approximately 20–50 ng/mL. For highly potent drugs, CV methods to reach sensitivity levels of low ng/mL can be implemented using UV...

Liquid chromatography coupled with tandem mass-spectrometry has remarkably enhanced the qualitative and quantitative analysis of pesticide residues in agriculture matrices. The matrix effects may either suppress or enhance responses target analytes on UPLC-MS/MS cause a reduction accuracy, precision sensitivity an analytical method. Thus, Here performed impact interferences tomato okra QuEChERS...

Purpose: To study the metabolites of phloretin in vivo using ultra-high performance liquid chromatography linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap).
 Methods: After administration (50 mg/kg; oral route) to six rats, blood samples were taken from each animal. Each sample was then subjected solid-phase extraction prepare it for chromatographic/spectroscopic analysis. Fi...