Two dimensional polyacrylamide gel electrophoresis (2D-PAGE) maps represent an unavoidable tool in many fields connected with proteome research, such as development of new diagnostic assays or new drugs. Unfortunately the information contained in the maps is often so complex that its recognition and extraction usually requires complex statistical treatments. Statistics accompanies many phases o...

The class of tensor product cubic splines is considered in an optimization process for two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) image alignment problems arising in proteomics studies. Numerical results are presented along with comparisons with previous results based on piecewise affine and bilinear transformations.

The goal of the two-dimensional (2D) electrophoresis protocol described here is to show how to analyse the phenotype of human cultured macrophages. The key role of macrophages has been shown in various pathological disorders such as inflammatory, immunological, and infectious diseases. In this protocol, we use primary cultures of human monocyte-derived macrophages that can be differentiated int...

Objective: Detection and evaluation of adrenal glands are complex problems in conventional ultrasonography in dogs. Using three-dimensional (3D) ultrasonography (US) in adrenal gland volume evaluation can add new potential for further adrenal studies. Design: Descriptive study Animals: a total of 10 mix breed dogs Procedures: The dogs underwent two dimensional (2D) and 3D US of the adrenal g...

‎‎‎In this paper‎, ‎we develop multi-step methods to solve a class of two-dimensional nonlinear Volterra integral equations (2D-NVIEs) of the first kind‎. ‎Here‎, ‎we convert a 2D-NVIE of the first kind to a one-dimensional linear VIE of the first kind and then we solve the resulted equation numerically by multi-step methods‎. ‎We also verify convergence and error analysis of the method‎. ‎At t...

Many protozoan parasites represent an important group of human pathogens. Pulsed Field Gradient Gel Electrophoresis (PFGE) analysis has been an important tool for fundamental genetic studies of parasites like Trypanosoma, Leishmania, Giardia or the human malaria parasite Plasmodium falciparum. We present PFGE conditions allowing a high resolution separation of chromosomes ranging from 500 to 40...

in this paper, a new design of optical channel drop filter based on two- dimensional photonic crystal ring resonators with triangular lattice is proposed. the rods of this structure is silicon with the refractive index nsi=3.46 and the surrounding environment is air with the refractive index of nair=1.the widest photonic band gap obtains for the filling ratio of r/a = 0.2. the filter’s transmis...

The present study aimed to compare the 2-dimensional (2D) electrophoresis pattern of the cerebrospinal fluid (CSF) in multiple sclerosis (MS), neuromyelitis optica (NMO) and control individuals, to identify the proteins with differential expression and to examine their significance. CSF samples from the three groups were collected and total protei...