BACKGROUND Genetic code expansion has developed into an elegant tool to incorporate unnatural amino acids (uAA) at predefined sites in the protein backbone in response to an amber codon. However, recombinant production and yield of uAA comprising proteins are challenged due to the additional translation machinery required for uAA incorporation. RESULTS We developed a microtiter plate-based hi...

The discovery that certain mutations result in the production of unfinished, amino-terminal peptide fragments of the corresponding wild-type proteins suggested that "nonsense" codons may normally signal termination of polypeptide chain synthesis.'-3 :\Ioreover, the finding that these "amber" mutations are suppressible, whereby the resulting polypeptide contains serine (codon, UCG) in place of g...

Solanum is a genus which belongs to Solanaceae family, includes many species of cropsor medicinal plants as eggplant, tomato, potato, twoleaf nightshade, etc. Number are used herbs in Vietnam up 41, one those procumbens Lour., often treat hepatitis or cirrhosis. The needs planting this plant rising up, but it still lacks studies based on DNA barcode Solanum, order prevent the confusion raw mate...

Analyses of ribosomal ITS and chloroplast trnL-F sequences provide phylogenetic reconstruction for the festucoids (Poeae: Loliinae), a group of temperate grasses with morphological and molecular affinities to the large genus Festuca. Parsimony and Bayesian analyses of the combined ITS/trnL-F dataset show Loliinae to be monophyletic but unresolved for a weakly supported clade of 'broad-leaved Fe...

Nucleotide sequence data from the chloroplast rbcL gene and the trnL-trnF intergenic spacer of 58 species in 38 genera were used to infer the phylogenetic affinities of Monimiaceae to other Laurales, and to assess whether the family in the traditional wide sense is monophyletic. Besides Monimiaceae, the Laurales comprise Calycanthaceae, Gomortegaceae, Hernandiaceae, and Lauraceae. Magnoliaceae ...

The presence of spliceosomal introns in eukaryotes raises a range of questions about genomic evolution. Along with the fundamental mysteries of introns' initial proliferation and persistence, the evolutionary forces acting on intron sequences remain largely mysterious. Intron number varies across species from a few introns per genome to several introns per gene, and the elements of intron seque...

Abstract A phylogeny based on the analysis of six DNA sequence markers (ITS, ndhA intron, rpl32‐trnL , rps3, rps16 and rps16‐trnK ) is used to infer ancestral areas divergence times, reconstruct biogeographical history evolution 150 183 (82%) species Muhlenbergia . Our results suggest that genus originated 9.3 mya in Sierra Madre (Occidental Oriental) Mexico, splitting into lineages: M. ramulos...

Leucobryum boninense is endemic to the Bonin Islands, Japan, and its related species are widely distributed in Asia and the Pacific. We aimed to clarify the phylogenetic relationships among Leucobryum species and infer the origin of L. boninense. We also describe the utility of the chloroplast trnK intron including matK for resolving the phylogenetic relationships among Leucobryum species, as p...

Mutations at position C1054 of 16S rRNA have previously been shown to cause translational suppression in Escherichia coli. To examine the effects of similar mutations in a eukaryote, all three possible base substitutions and a base deletion were generated at the position of Saccharomyces cerevisiae 18S rRNA corresponding to E. coli C1054. In yeast, as in E. coli, both C1054A (rdn-1A) and C1054G...