conclusions the obtained results indicate that rsm is an effective method for the optimization of culture conditions for hes gene expression in e. coli. results a maximum rhes mrna level of 376.16% was obtained under the following conditions: 0.6 mm iptg, 7 hours post-induction time, and 0.9 cell density before induction. the level of rhes mrna was significantly correlated with post-induction t...

objective: to evaluate the effect of cytoplasm transfer from mature oocytes to germinal vesicle(gv)s on promoting the maturation of cytoplasm of gv at the mrna level. materials and methods: sixty six in vitro fertilization (ivf) operations between june 2012 and november 2013 were included in this study. totally 120 gvs were obtained. normal gvs were categorized into 3 groups (n = 40) randomly: ...

background: influenza virus is the major cause of lower respiratory tract illnesses on the worldwide. vaccination can be an effective tool to prevent its outbreak. highly conserved viral nucleoprotein is an effective vaccine candidate to provide heterosubtypic immunity, offering resistance against various influenza virus strains. m aterials and methods: in present research np gene was inserted ...

background: with the increasing concern for health and nutrition, dietary fat has attracted considerable attention. the composition of fatty acids in the diet is important because they are associated with major diseases including cancers, diabetes, and cardiovascular disease. the fatty acid synthase (fasn) and stearoyl-coa desaturase (delta-9-desaturase) (scd) genes affect fatty acid compositio...

background: as the third most frequent cause of cancer death, breast cancer is a common disease worldwide. most of the patients are being diagnosed in the stage that conventional treatments are not effective, and invasion and metastases lead to death. therefore, identification of novel molecular markers to improve early diagnosis, prognosis and treatment of the breast cancer is a necessity. zin...

background: recombinant vaccine technology is one of the most developed means in controlling infectious diseases. however, an effective vaccine against shigella is still missing. objective: to evaluate recombinant ipac protein of shigella as a vaccine candidate. methods: in this study we cloned ipac gene into an expression vector in prokaryotic system. the protein expression was evaluated by sd...

background: the impact of cadmium (cd) on male infertility may be related to the interaction with metal-binding proteins known as metallothioneins (mts). trace elements like zinc (zn) have protective effects on testicular damage induced by cd. objective: we determined the effect of zn and low-dose cd pre-treatment on the expression of mt1 and mt2 genes on testicular sertoli cells. materials and...

bovine ephemeral fever (bef) is an acute epidemic disease in cattle and water buffalo, spanning tropical and subtropical zones of asia, australia, and africa. in recent years, bef has been distributed in many provinces of iran and caused economic losses. in this study the sequence encoding the g1 epitope of bovine ephemeral fever virus (befv) g glycoprotein was amplified by pcr, ligated into th...