Although miR-5195-3p has been acknowledged for its tumor suppressor role in diverse cancer categories, precise functions and mechanisms concerning melanoma have not comprehensively elucidated. In this study, we employed quantitative reverse transcription PCR, Western blot analysis, immunohistochemistry staining to investigate the expression patterns of poly (rC) binding protein 2 (PCBP2) tissue...

Two major intracellular signals that regulate neuronal function are calcium and cAMP. In many cases, the actions of these two second messengers involve long term changes in gene expression. One well studied target of both calcium and cAMP signaling is the transcription factor cAMP-responsive element-binding protein (CREB). Multiple signaling pathways have been shown to contribute to the regulat...

The balanced growth of a cell requires the integration of major systems such as DNA replication, membrane biosynthesis, and ribosome formation. An example of such integration is evident from our recent finding that, in Saccharomyces cerevisiae, any failure in the secretory pathway leads to severe repression of transcription of both rRNA and ribosomal protein genes. We have attempted to determin...

Transcriptional activation often requires the rapid assembly of complexes between dimeric transcription factors and specific DNA sites. Here we show that members of the basic region leucine zipper and basic region helix-loop-helix zipper transcription factor families follow an assembly pathway in which two protein monomers bind DNA sequentially and form their dimerization interface while bound ...

Purpose: Data on microarray gene expression The Gene Expression Omnibus (GEO) provided information expression. Transcription GEO two profiles of human NK cells from breast and adrenal tumors (GSE179509 GSE143383). processing normalization Dseq2 tool in the R programming language was used to standardize raw data GEO. following analyses were carried out: fold change P-value analysis, volcano plot...

Paracrine Hedgehog (Hh) signaling regulates growth and patterning in many Drosophila organs. We mapped chromatin binding sites for Cubitus interruptus (Ci), the transcription factor that mediates outputs of Hh signal transduction, and we analyzed transcription profiles of control and mutant embryos to identify genes that are regulated by Hh. Putative targets that we identified included several ...

DNA replication stress can cause chromosomal instability and tumor progression. One key pathway that counteracts replication stress and promotes faithful DNA replication consists of the Fanconi anemia (FA) proteins. However, how these proteins limit replication stress remains largely elusive. Here we show that conflicts between replication and transcription activate the FA pathway. Inhibition o...

Seed germination is essential for direct seeding in rice. It has been demonstrated that trehalose-6-phosphate phosphatase 1 (OsTPP1) plays roles improving yield and stress tolerance In this study, the of OsTPP1 on seed rice were investigated. The tpp1 mutant germinated slower than wild-type (WT), which can be restored by exogenous trehalose. seeds showed higher ABA content compared with WT seed...

Hundreds of different proteins regulate and implement transcription in Saccharomyces. Yet their interrelationships have not been investigated on a comprehensive scale. Here we determined the genome-wide binding locations of 200 transcription-related proteins, under normal and acute heat-shock conditions. This study distinguishes binding between distal versus proximal promoter regions as well as...