A longitudinal study of different parameters of the immune responses to Toxoplasma gondii was performed with sera of AIDS patients taken during and after clinically important Toxoplasma infections. Follow-up of patients lasted for 9 months on an average. The titres of the specific IgG and IgM antibodies were measured by an indirect fluorescent antibody test (IFAT), and the appearance of circula...

PURPOSE To describe a case of recurrence of congenital ocular toxoplasmosis with frosted branch angiitis. CASE REPORT A 24-year-old woman presented with hyperemia in her right eye. Medical history included epilepsy at age 14 and mild mental retardation. Iridocyclitis and vitreous opacity were observed in the right eye, and furthermore widespread retinal vessel sheathing due to frosted branch ...

A recently developed enzyme-linked immunosorbent assay for detection of immunoglobulin M (IgM) class antibodies to Toxoplasma gondii was evaluated with respect to specificity and sensitivity. By using an antibody capture principle and F(ab')2 conjugates, interference of rheumatoid factors was absent. No cross-reactions with anti-toxoplasma IgG occurred, and no interference with antinuclear anti...

A dipstick dye immunoassay (DDIA) was developed to detect immunoglobulin G (IgG) or IgM antibodies of toxoplasmosis infection in humans. The assays employ a blue colloidal dye particles (D-1) conjugated to sheep anti-human IgG and rabbit anti-human IgM as the visualizing agents and a soluble antigen of tachyzoites of Toxoplasma gondii strain RH (TSA) as the detective antigen. The mixture of dye...

OBJECTIVE Ivermectin and sulphadiazine were tested individually to determine their in vitro effects on Toxoplasma gondii grown in human epidermoid larynx carcinoma (Hep-2) cell culture. STUDY DESIGN In-vitro study. MATERIAL AND METHODS Toxoplasma growth was quantities by an enzyme immunoassay performed directly on the fixed cultures, using a rabbit anti-T. gondii immunoglobulin G as the fir...

objective: toxoplasmosis may cause significant damage to the developing fetus and is a life-threatening opportunistic infection in immunocompromised persons. molecular methods are known to be more sensitive and more specific than serological assays for diagnosis of toxoplasmosis. application of quantitative pcr has evolved sensitive, specific, and rapid method for the detection of rh strain of ...

Background and aims: Toxoplasmosis is a very common disease in the world. Two types of acquiredtoxoplasmosis have been detected. In the chronic toxoplasmosis, the abnormality of tissue functionis little but, in acute toxoplasmosis, function of RES system becomes interrupted. The assessment oftoxoplasma antibody with tissue enzymes in this stage is very important. Furthermore, ...

Objective: The aim of this study was to determine the seroprevalence Toxoplasma gondii (T. gondii) in schizophrenia patients and reveal importance toxoplasmosis as a risk factor these patients. Method: conducted on 190 diagnosed with schizophrenia. Also included control group were 100 healthy individuals without Anti-T. immunoglobulin G (IgG) IgM antibody levels determined via enzyme-linked imm...

In a cross-sectional study, we evaluated the seroprevalence of Toxoplasma gondii among 400 women referred to Qazvin community health centre laboratory for pre-marriage examinations. Indirect immunofluorescent antibody test was used to detect IgG anti-toxoplasma. Titres > or = 1: 20 were considered positive. The overall seropositivity was 34%. Mean age was significantly higher in seropositive wo...

Blood inoculation in mice showed that Toxoplasma organisms circulate in blood after 1 h of oocyst infection. Parasites were detected up to 15 days later and then disappeared from the bloodstream concomitantly with cyst formation in the brain, probably due to antibody presence. Immunosuppression caused by cortisone acetate treatment induced Toxoplasma bloodstream invasion in chronically infected...