Purified m beta-acrosin catalysed amidolysis of several p-nitroanilides with C-terminal arginine residues. Antithrombin III inhibited amidolysis catalysed by the enzyme. This effect of antithrombin III was potentiated by heparin, and to a modest extent by heparan sulphate, cellulose sulphate, dextran sulphate and xylan sulphate. De-N-sulphated heparin, de-N-sulphated N-acetylated heparin, hepar...

the aim of the study was to evaluate the antioxidant and free radical scavenging properties and to determine the phenolic content of the ethanol extract from five mentha species [m. longifolia (l.) huds., m. piperita l., m. pulegium l., m. rotundifolia (l.) huds., and m. spicata l.]. the antioxidant activities of the extracts were investigated with two different methods, 2,2’-diphenyl-1-picrylh...

the objective of this research was to study the biological activities of various extracts and the chemical composition of the essential oil trigonella monantha c. a. mey. subsp. monantha by gas chromatography (gc) and gas chromatography-mass spectroscopy (gc-ms), from the compounds derived from the aerial parts. the overall results of t. monantha tests allow us to conclude various extracts [hex...

introduction : in the recent years, due to the wide spread of resistant bacteria on one side and several different reports about the side effects of chemical drugs on the other side, vast researches on the medicinal plants have been started. in this study, antimicrobial effect of total extract of tribulus terrestris l . and its fraction containing benzoxazine derivative (terresoxazine) was stud...

The mouse zona pellucida glycoprotein, mZP2, is thought to be the secondary receptor on eggs for retention of acrosome-reacted sperm during fertilization. Here, we present evidence that one of its complementary binding proteins on sperm is proacrosin/acrosin. mZP2 binds to proacrosin null sperm considerably less effectively than to wild-type sperm. Binding is mediated by a strong ionic interact...

Hyaluronidase, acrosin and N-acetylhexosaminidase activities were examined in sperm collected from 12 beagle dogs and in culture medium after 0.5 hr and 7 hr of sperm incubation. The activities of the three enzymes were significantly higher at 7 hr than at 0.5 hr (P < 0.05, 0.01), and the increases were associated with sperm capacitation. It was considered that the three enzymes in the dog sper...

The current study investigated the relationship between the level of expression and tyrosine phosphorylation of the sperm protein 32 (sp32) and the activation of the boar proacrosin/acrosin system. The acrosomal membrane proteins of boar sperm for use in different treatment experiments (i.e., fresh sperm, freezing and thawing, capacitation, and acrosome reaction) were separated, stained by Coom...