Ornithine decarboxylase (ODC) is a cytosolic enzyme that catalyses the direct decarboxylation of L-ornithine to putrescine, one of the rate-limiting steps of polyamine biosynthesis in plants. We targeted recombinant human ODC to the cytosol and apoplast of transformed tobacco (Nicotiana tabacum) plants, and evaluated the impact of subcellular compartmentalization on the accumulation of the enzy...

Glutamate decarboxylase (GAD, EC 4.1.1.15) catalyses the alpha-decarboxylation of glutamate to produce gamma-aminobutyrate (GABA). The nucleotide sequences of two divergent GADs (designated GAD1 and GAD3) were isolated from a Nicotiana tabacum L. cv. Samsun NN leaf cDNA library. Open reading frames indicated that GAD1 encodes a polypeptide of 496 amino acids and has greater than 99% identity wi...

A 1312 bp 5' flanking region of Salicornia europaea choline monooxygenase (SeCMO) gene was isolated using the anchored PCR. To investigate the mechanism of regulation for this stress-induced gene, the SeCMO promoter-beta-glucuronidase (GUS) chimeric gene constructs containing five deletions F1, F2, F3, F4 and F5 were introduced into tobacco (Nicotiana tabacum L.) by Agrobacterium-mediated trans...

In the yeast two-hybrid screening of plant factors interacting with tobacco mosaic virus (TMV) RNA-dependent RNA polymerase (RdRp), we found a protein containing a RING finger motif in tobacco (Nicotiana tabacum) and designated it as TARF (TMV-associated RING finger protein). TARF is a homologue of a Lotus japonicus RING finger protein (LjnsRING) involved in the symbiotic interaction between L....

Tobacco (Nicotiana tabacum L.) is an important industrial crop grown in more than one hundred countries worldwide, with high adaptability on a great variety of soils, and under quite diverse climatic conditions. Information regarding the chemical composition tobacco leaves for industry because it generally may exhibit differences among different locations. In this study, five oriental six flue ...

Interspecific amphihaploid and amphidiploid hybrids between Nicotiana glauca Grah. (2n = 24) and N. tabacum L. (2n = 48) cultivars BY 103 and K 326 were analysed. F1 amphihaploids (2n = 36) were viable and completely self- and cross-sterile, and mostly univalents were present during meiosis (with pairing range from 0 to 5). In some meiocytes, meiotic irregularities were observed, such as sporad...

the influenza a virus is of global concern for the poultry industry, especially the h5 subtype as it has the potential to become highly pathogenic for poultry and mankind. recently, plant expression systems have gained interest as an alternative for the production of vaccine antigens. the goal of the present study was to investigate the possibility of expressing the ha1 protein in nicotiana tab...