One of the limitations of conventional tissue culture on flat two-dimensional surfaces is the loss of complex interactions between the epithelium and stroma. We have devised a culture system that recreates the salient features of the stratified epithelium using primary cell cultures from mouse models. The protocol described here is applicable to the esophageal epithelium, but stratified epithel...

The identification of a widespread contaminant of plant cell cultures is reported. Mycobacterium scr~luhtceum Prissick and Masson appears as yellow colonies on the medium and plant tissues of many ornamentals being propagated in vitro. It is a Gram positive and acid fast bacterium and requires a specifically formulated medium for growth in pure culture. Many laboratories have alluded to this co...

Adherent mammalian cells dynamically interact with their extracellular matrix (ECM) and culture substrate. To accommodate this sensitivity, standard culture techniques typically utilize tissue culture polystyrene (TCPS), a treated polystyrene substrate that promotes cell attachment. However, TCPS cannot be easily integrated into microfluidic devices as it is incompatible with conventional fabri...

Integrin- and cadherin-mediated adhesion is central for cell and tissue morphogenesis, allowing cells and tissues to change shape without loosing integrity. Studies predominantly in cell culture showed that mechanosensation through adhesion structures is achieved by force-mediated modulation of their molecular composition. The specific molecular composition of adhesion sites in turn determines ...

Here, we present a novel method for culturing kidneys in low volumes of medium that offers more organotypic development compared to conventional methods. Organ culture is a powerful technique for studying renal development. It recapitulates many aspects of early development very well, but the established techniques have some disadvantages: in particular, they require relatively large volumes (1...

Tissue culture has been used for the study of animal viruses since 1906 when Aldershoff and Boers first grew vaccinia in rabbit tissue separate from the whole host.2 Other limited successes were obtained in the years following, but extensive use of tissue culture in animal virology awaited the studies of Enders, Weller, and Robbins' on a human agent, poliovirus. Since then, the use of tissue cu...

Even though the physiological role of estrogen in the female reproductive cycle and endometrial proliferative phase is well established, the signaling pathways by which estrogen exerts its action in the endometrial tissue are still little known. In this regard, advancements in cell culture techniques and maintenance of endometrial cells in cultures enabled the discovery of new signaling mechani...

Adult hippocampal neural progenitor cell (AHNPC) culture is a useful technique for gaining insight into adult neurogenesis, studying disease, and high throughput drug screening. The ability of AHNPCs to proliferate and differentiate into the three cell lineages of the adult brain in cell culture provides the researcher a powerful platform to study the extracellular and intracellular regulatory ...

Despite the successful transfer of mammalian in vitro techniques for use with fish and other vertebrates, little progress has been made in the area of invertebrate tissue culture. This paper describes the development of an in vitro technique for the culture of both cells in suspension and tissue explants from the gill, digestive gland and mantle of the zebra mussel (Dreissena polymorpha) and th...

Strawberry cultivation is not popular in Bangladesh due to the unpredictable climatic conditions and lack of proper cultivars. Using somaclonal variation, several new promising selections were generated and evaluated for their flowering and fruiting ability, adaptability and sustainability. To induce variation, plants were regenerated using various tissue culture techniques. Our results suggest...