The cytoskeleton plays several fundamental roles in the cell, including organizing the spatial arrangement of subcellular organelles, regulating cell dynamics and motility, providing a platform for interaction with neighboring cells, and ultimately defining overall cell shape. Fluorescence imaging has proved to be vital in furthering our understanding of the cytoskeleton, and is now a mainstay ...

This review provides an overview of digital radiography as it exists, including advanced imaging such as computed tomography (CT), cone beam volumetric imaging, and micro-CT as relevant to the practice of endodontics. An evidence-based approach to adoption of different imaging technologies is included to assist the practitioner with the selection process of imaging modalities. Commonly used ima...

We show the applicability of 4Pi-confocal microscopy to three-dimensional imaging of the microtubule network in a fixed mouse fibroblast cell. Comparison with two-photon confocal resolution reveals a fourfold better axial resolution in the 4Pi-confocal case. By combining 4Pi-confocal microscopy with Richardson-Lucy image restoration a further resolution increase is achieved. Featuring a three-d...

We developed two-photon scanning patterned illumination microscopy (2P-SPIM) for super-resolution two-photon imaging. Our approach used a traditional two-photon microscopy setup with temporally modulated excitation to create patterned illumination fields. Combing nine different illuminations and structured illumination reconstruction, super-resolution imaging was achieved in two-photon microsco...

In purely repulsive colloidal systems a glass transition can be reached by increasing the particle volume fraction beyond a certain threshold. The resulting glassy state is governed by configurational cages which confine particles and restrict their motion. A colloidal glass may also be formed by inducing attractive interactions between the particles. When attraction is turned on in a repulsive...

The analysis of shape is a key part of anatomical research and in the large majority of cases landmarks provide a standard starting point. However, while the technology of image capture has developed rapidly and in particular three-dimensional imaging is widely available, the definitions of anatomical landmarks remain rooted in their two-dimensional origins. In the important case of the human f...

We present a method to directly image fluorescent structures inside turbid media. This is based on wave-front shaping to optimize the scattered light onto a single fluorescent particle, as the optical memory effect for a scanning image of the surroundings of this particle. We show that iterating the optimization leads to the focusing on a single particle whose surroundings are subsequently scan...

In this paper, we present a new method for face recognition using range data. The proposed method is based on both global statistics of geometrical features and local statistics of correlative features of facial surfaces. Firstly, we analyze the performances of common geometrical representations by using global histograms for matching. Secondly, we propose a new method to encode the relationshi...

geboren te Breda. This work was carried out in the ASCI graduate school. ASCI dissertation series number 194.

Optical impulse-response characterization of diffusive media can be of importance in various applications, among them optical imaging in the security and medical fields. We present results of an experimental technique that we developed for acquiring the impulse response, based upon the Kramers-Kronig algorithm, and have been applied for optical imaging of objects hidden behind clothing. We demo...