Prepara t ive separation of bile acids has been achieved by adsorption and partition chromatography, and microscale separations have been made by various paper-chromatographic methods (1). Using these methods, good separations and acceptable sensitivity can be achieved. Methods are lacking, however, that permit rapid qualitative analysis of a large number of samples; e.g., for the monitoring of...

With regard to the liquid chromatographic methods described for bile acid analysis, especially with regard to their determination in biological matrices, several approaches for qualitative and quantitative determination have been described. A critical evaluation of the advantages and disadvantages of these methods results in the conclusion that there are speciRc limitations for every applicatio...

Crude ether extracts of green shoots of Cucumis sativus L. promoted the elongation of cucumber hypocotyl segments. Purification of the extract was accomplished by DEAE cellulose, silicic acid, and magnesium silicate chromatography followed by gel filtration and preparative thin layer chromatography. Identification of the growth promoter as indole-3-ethanol was achieved by mass spectrometry, thi...

The carotenoids of yellow, red and tangerine tomato fruits were separated and identified by thin-layer chromatography (TLC). β-carotene is extracted with a mixture content hexan and acetone. The various systems of adsorbents and solvents for TLC of carote and tomato carotenes were examined, and it was revealed that the most suitable TLC system for separation and identification was silica gel pl...

The use of impregnated phases in thin-layer chromatography (TLC) has a long history, beginning in the very earliest days of the technique. As a consequence, much of the key literature in this Reld dates back to the 1960s and 1970s. The literature also contains a considerable number of examples of impregnation reagents where subsequently the methods have received little attention except in revie...

DURING A STUDY on the metabolism of histidine in a child affected with histidinemia (Beauvais, P., and Humbel, R., to be published), we became interested in the chromatography of imidazoles. In this disease the urine contains numerous imidazoles: histidine and its metabolites, imidazole-pyruvic, imidazole-lactic and imidazole-acetic acids and N-acetylhistidine.’I developed a chromatographic met...