Unusual thermostable and insensitive high-energy compounds which contain dioximes with donor–acceptor H-bond sites, were synthesized from glyoxal (40% in water) straightforward steps.

Aspergillus fumigatus was grown on chopped wheat straw in a solid state fermentation (SSF) process carried out in constant presence of isolated free water inside the fermentation chamber. The system allowed maintaining a constant vapor pressure inside the fermentor throughout the fermentation process. Crude endoglucanase produced by A. fumigatus under such conditions was more thermostable than ...

Problem statement: This study reported the purification and characterization of a novel highly thermostable alkaline amylase from a newly isolated Bacillus strain HUTBS71. Approach: The enzyme was purified using ammonium sulfate precipitation, ion exchange and gel filtration chromatography. Results: Maximum amylase activity (72 U mL) was obtained at 100°C after 10 min of incubation. The enzyme ...

A variety of serovars of the food-borne pathogen Vibrio parahaemolyticus normally cause infection. Since 1996, the O3:K6 strains of this pathogen have caused pandemics in many Asian countries, including Taiwan. For a better understanding of these pandemic strains, the recently isolated clinical O3:K6 strains from India, Japan, Korea, and Taiwan were examined in terms of pulsed-field gel electro...

This study proposes a thermostable refractive index (RI) sensor consisting of a silica microsphere coated with a poly(methyl methacrylate) (PMMA) layer. The first-order and second-order whispering gallery modes (WGMs) of both TE and TM polarizations are considered theoretically. The layer thickness is carefully optimized to eliminate the thermal drift and enhance the RI sensitivity and detectio...

Lipases (triacylglycerol acylhydrolase, EC 3.1.1.3), catalyze the hydrolysis of long-chain triglycerides with the formation of diacylglyceride, monoglyceride, glycerol and fatty acids. Lipases are actively used in various industries which include food and diary, pharmaceuticals, organic synthesis and detergent and cosmetics. Thermostable lipases are commercially significant for their potential ...

Hydrogen peroxide is widely used for chemical treatment in various industries particularly in the textile, paper, pulp, food and dairy industries as a more environmentally-friendly alternative to chlorine that forms toxic and carcinogenic chemicals called dioxins as by-products7,9,33). However, the rapid formation of reactive oxygen species (ROS) from hydrogen peroxide, including hydroxyl radic...

Rational shift of the optimum pH toward alkalinity and enhancement of thermostability were investigated by using a thermostable extremely alkaline protease (optimum pH, 12 to 13) from the alkaliphilic and thermophilic Bacillus sp. strain B18'. The protease gene (aprM) was cloned, and the sequence analysis revealed an open reading frame of 361 amino acids that was composed of a putative signal s...