The enzyme nicotinamide mononucleotide (NMN) adenylyltransferase (EC 2.7.7.1) catalyzes the synthesis of NAD+ and nicotinic acid adenine dinucleotide. It has been purified to homogeneity from cellular extracts of the thermophilic archaeon Sulfolobus solfataricus. Through a database search, a highly significant match was found between its N-terminal sequence and a hypothetical protein coded by t...

Hydrogen-producing thermophilic cellulolytic microorganisms were isolated from cow faeces. Rates of cellulose hydrolysis and hydrogen formation were 0.2 mM L(-1) h(-1) and 1 mM L(-1) h(-1), respectively. An enzymatic fuel cell (EFC) with a hydrogenase anode was used to oxidise hydrogen produced in a microbial bioreactor. The hydrogenase electrode was exposed for 38 days (912 h) to a thermophili...

The thermophilic, anaerobic, propionate-oxidizing bacterial populations present in the methanogenic granular sludge in a thermophilic (55 degrees C) upflow anaerobic sludge blanket reactor were studied by cultivation and in situ hybridization analysis. For isolation of propionate-degrading microbes, primary enrichment was made with propionate as the sole energy source at 55 degrees C. After sev...

The present study aimed to isolate soil microorganisms-degrading plant latices and to screen their capablilities of producing protease and lipase. Many bacterial and fungal strains of mesophilic, thermotolerant or thermophilic nature were able to grow on sugar-free medium amended with crude latex of either Euphorbia pulcherrima, Ficus elastica or F. nitida and incubated at 25oC, 36oC or 45oC. T...

Anaerobic digestion of sewage sludge (SS) and co-digestion with cheese whey (CW) was evaluated in batch conditions under mesophilic and thermophilic regimens. Results indicated that the co-digestion can be successfully attained despite some inhibitory stages that may be initially present due to the accumulation of volatile fatty acids. Degradation of the mixture SS-CW followed a similar pattern...

Two anaerobic acidogenic reactors, one mesophilic (37 8C) and one thermophilic (55 8C), were operated with a synthetic wastewater at a series of organic loading rates (OLRs). There was almost no difference between the two reactors for chemical oxygen demand (COD) reduction, and degree of acidification at any given OLRs. However, the thermophilic reactor had a higher substrate degradation rate, ...

The thermophilic streptococci are dominant members of the microflora of several types of cheese produced with a natural starter. A total of 205 isolates of thermophilic streptococci were obtained from 70 samples of white cheese which had been collected from producing areas of different regions of Syria during 2009 and 2010. The isolates were cocci gram positive and catalase negative. From these...

Hot springs have been investigated since the XIX century, but isolation and examination of their thermophilic microbial inhabitants did not start until the 1950s. Many thermophilic microorganisms and their viruses have since been discovered, although the real complexity of thermal communities was envisaged when research based on PCR amplification of the 16S rRNA genes arose. Thereafter, the pos...