نتایج جستجو برای: thermophile
تعداد نتایج: 943 فیلتر نتایج به سال:
Ancestral amino acid residues were inferred for 3-isopropylmalate dehydrogenase (IPMDH), and were introduced into the enzyme of an extreme thermophile, Sulfolobus sp. strain 7. The thermostability of the mutant enzymes was compared with that of the wild type enzyme. At least five of the seven mutants tested showed higher thermal stability than the wild type IPMDH. The results are compatible wit...
Synthetic biology applications rely on a well-characterized set of microbial strains, with an established toolbox molecular methods for their genetic manipulation. Since there are no thermophiles such attributes, most biotechnology and synthetic studies use organisms that grow in the mesophilic temperature range. As result, thermophiles, heterogenous group microbes thrive at high (>50 °C) te...
Mutants of the extreme thermophile Thermus flavus in the pyrimidine biosynthetic pathway (Pyr-) were isolated by resistance to 5-fluoroorotic acid. The pyrE gene, which codes for the orotate phosphoribosyltransferase, was cloned by recombination with one of the isolated Pyr- T. flavus mutant strains. It was subcloned by complementation of an Escherichia coli pyrE mutant strain and was sequenced...
Here, a low-cost glucose/O 2 Y-shaped microfluidic biofuel cell was developed using printed circuit board for microelectrode construction. A double-side tape based on the pressure-sensitive adhesive used microchannel fabrication. nanocomposite that consisted of reduced graphene oxide gold nanoparticles (AuNPs), and poly neutral red connected to enzymes applied copper electrode surface. The Aspe...
Aqualysin I is the alkaline serine protease isolated from an extreme thermophile, Thermus aquaticus YT-1. We analyzed kinetic properties of aqualysin I, using sixteen kinds of chromogenic succinyl-tripeptide p-nitroanilides as substrates. And we compared the substrate specificity of aqualysin I with those of proteinase K, subtilisin BPN', and subtilisin Carlsberg. We found that aqualysin I had ...
DNA from low-biodiversity fracture water collected at 2.8-kilometer depth in a South African gold mine was sequenced and assembled into a single, complete genome. This bacterium, Candidatus Desulforudis audaxviator, composes >99.9% of the microorganisms inhabiting the fluid phase of this particular fracture. Its genome indicates a motile, sporulating, sulfate-reducing, chemoautotrophic thermoph...
Tween 80 (0.1%, v/v) added to Thermomonospora curvata growing in minimal medium caused a transient lowering of the dry cell mass, decreased the optimal growth temperature of the thermophile from 62 to 54°C, and increased extracellular esterase activity. Cells grown in the presence of Tween 80 had decreased concentrations of branched chain fatty acids and increased concentrations of oleic acid. ...
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