The mechanism by which phagocytosed mast cell granules (MCGs) inhibit macrophage superoxide production has not been defined. In this study, rat peritoneal macrophages were co-incubated with either isolated intact MCGs or MCG-sonicate, and their respiratory burst capacity and morphology were studied. Co-incubation of macrophages with either intact MCGs or MCG-sonicate resulted in a dose-dependen...

OBJECTIVE To investigate the potential protective effects of amitriptyline and fluoxetine in a catecholamine cell model. METHODS Cultured rat pheochromocytoma (PC12) cells were pretreated with amitriptyline or fluoxetine for 24 or 48 hours and were then subjected to neurotoxic insult (200 micromol/L hydrogen peroxide). Cell viability was determined by measurement of the reduction product of 3...

objective(s): neuroinflammation and oxidative stress play a key role in pathogenesis of parkinson’s disease (pd). in the present study we investigated the effect of reactive oxygen species (ros) scavenger wr-1065 on catalepsy and cerebrospinal fluid (csf) level of interleukin 6(il-6) and striatum superoxide dismutase (sod) activity  in 6-hydroxydopamine (6-ohda) induced experimental model of pd...

BACKGROUND Isoniazid (INH) is front-line anti-tuberculosis (TB) drugs, which are usually prescribed to TB patients for a total period of 6 months. Antituberculosis drug-induced hepatotoxicity (ATDH) is a serious adverse reaction of TB treatment. It is reported that INH-induced hepatotoxicity is associated with oxidative stress. Superoxide dismutase (SOD, EC 1.15.1.1) is the key enzyme for the p...

BACKGROUND The aim of this study was to compare superoxide dismutase (SOD) activity in Fasciola hepatica and Fasciola gigantica parasites. MATERIALS F. gigantica and F. hepatica helminths were collected from abattoir and cultured in buffer media for 4 h at 37 °C. Excretory-Secretory (ES) products were collected, centrifuged and stored at -20(°)C. E-S protein concentration was measured by Brad...

one of the major classes of ionotropic glutamate receptors is the class of n-methyl-d-aspartate receptors (nmdars). receptor activation recruits, via calcium signal transduction mechanisms which play important roles in oxidative metabolism, mitochondrial free radical production and occurrence of other mitochondrial factors which potentially contribute to excitotoxicity and neuronal death. in...

The secretory enzyme extracellular-superoxide dismutase (EC-SOD) has affinity for heparin and some other sulfated glycosaminoglycans and is in vivo bound to heparan sulfate proteoglycan. Nonenzymic glycation of EC-SOD, both in vivo and in vitro, is associated with a reduction in heparin affinity, whereas the enzymic activity is not affected. The glycation sites in EC-SOD are further studied in ...

A potted experiment was carried out to study the effect of an arbuscular mycorrhizal fungus (Diversispora versiformis) and like (Piriformospora indica) on antioxidant enzyme defense system Satsuma orange (Citrus sinensis cv. Oita 4) grafted Poncirus trifoliata under favourable temperature (25°C) cold (0°C) for 12 h. Such short-term treatment did not cause any significant change in root fungal c...