Degradative strains of fast-growing Mycobacterium spp. are commonly isolated from polycyclic aromatic hydrocarbon (PAH)-contaminated soils. Little is known, however, about the ecology and diversity of indigenous populations of these fast-growing mycobacteria in contaminated environments. In the present study 16S rRNA genes were PCR amplified using Mycobacterium-specific primers and separated by...

A two-dimensional separation system is presented combining scanning temperature gradient focusing (TGF) and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) in a PDMS/glass microfluidic chip. Denatured proteins are first focused and separated in a 15 mm long channel via TGF with a temperature range of 16-47 °C and a pressure scanning rate of -0.5 Pa/s and then further sepa...

Sequence-specific recognition of DNA can be achieved by triple helix-forming oligonucleotides that bind to the major groove of double-helical DNA. These oligonucleotides have been used as sequence-specific DNA ligands for various purposes, including sequence-specific gene regulation in the so-called 'antigene strategy'. In particular, (G,A)-containing oligonucleotides can form stable triple hel...

We present an online tool (EquiBands, http://www.univie.ac.at/IECB/limno/equibands/EquiBands.html) that quantifies the matching of two bands considered to be the same in different samples, even when samples are applied to different denaturing gradient gel electrophoresis gels. With an environmental example we demonstrate the procedure for the classification of two bands of different samples wit...

and -IgM k and l light chains did not show the presence of monoclonal components. Reduction with mercaptoethanol (1:2 dilution), which dissociates complexes but does not dissociate albumin dimers, excluded the possible formation of complexes with a1-antitrypsin. Interferences by radio-opaque agents or medications, which could lead to the appearance in the CZE electropherograms of abnormal peaks...

Analysis of microbial communities associated with plants, insects and soil has been a significant challenge for plant protection researchers, because of the lack of techniques with which to access these populations. A new molecular community profiling technique, 16S rRNA gene-based PCR followed by denaturing gradient gel electrophoresis (DGGE), has been used to analyse microbial communities in ...