MicroRNAs (miRNAs) are incorporated into miRNP complexes and regulate protein expression post-transcriptionally through binding to 3'-untranslated regions of target mRNAs. Here we describe a recapitulation of let-7 miRNA-mediated translational repression in a cell-free system, which was established with extracts prepared from HEK293F cells overexpressing miRNA pathway components. In this system...

matococcus daniellii Benth.i) The moleculaT mass of the protein is 22 kDa and the higher order structure of the protein is maintained by eight disulfide bridges.2・3) Thaumatin is the sweetest substance for man and is, on a molar basis, 100,OOO times sweeter than sucrose.i) Today, thaumatin is used commercially for its intense sweetness, taste masking, fiavor enhancement, and synergistic propert...

Mutations in the genes for two highly conserved TAFs, TAF(II)60 and TAF(II)110, reduce transcription of Bicoid-dependent target genes in vivo. By means of several distinct genetic test systems, specific activator-TAF interactions are shown to support both simple and synergistic enhancement of transcription in the embryo. These studies provide in vivo evidence that TAFs can serve as coactivators...

alismatis rhizoma decoction (ard) is a classical traditional chinese medicine (tcm) formula for treatment of vertigo with its long history of successful clinical effect. since vertigo is a symptom of hyperlipidemia, this study aimed at evaluating the hypolipidemic effect of ard in hyperlipidemia mice induced by high fat diet (hfd) and investigated the rationality of formula combination of alism...

objective: depression is a major health problem, which is not only underrecognized and undertreated, but is also associated with significant morbidity and mortality. it has been suggested that combination therapy rapidly reduces depressive symptoms in patients with moderate to severe depression and is more effective than monotherapy; but this suggestion remains controversial. serotonergic and n...