The evoked effects of the negatively charged drugs phenobarbital and barbituric acid, the positively charged imipramine, perphenazine and trifluoperazine, and the neutral primidone, on the synaptosome-associated acetylcholinesterase activity were studied. A marked increase in the enzyme activity was exhibited in the presence of low concentrations (up to 3 mM) of phenobarbital, barbituric acid a...

A biochemical and quantitative morphologic study of presynaptic endings during postnatal development was carried out in subcellular fractions from cerebral cortex of 1, 4, 8, 12, and 18 day old and adult rats. Crude mitochondrial fractions were subfractionated in Ficoll gradients and all resulting fractions were examined in the electron microscope. Presynaptic terminals and other intact process...

The aim of this study was to explore the pivotal genes or lncRNAs involved in progression atrial fibrillation (AF) -valvular heart disease (VHD). mRNA profiling GSE113013 obtained from Gene Expression Omnibus database. identification differentially expressed (DEGs) and long non-coding RNAs (DElncRNAs) performed. Ontology Kyoto Encyclopedia Genes Genomes pathway enrichment analyses were carried ...

In the present experiments we have measured the liberation of [‘4C]adenosine derivatives and lactate from ‘synaptosome beds’ (de Belleroche & Bradford, 1972), prelabelled with [8-14C]adenosine and superfused with glucose/bicarbonate medium with or without metabolic inhibitors and depolarizing agents. To interpret our findings we have also measured ATP, ADP and AMP of the superfused synaptosomes...

presence of rotenone and oligomycin (Scott & Nicholls, 1980). This combination of inhibitors should prevent any Ca’+ uptake into the matrices of the mitochondria. As is shown in Table 1, the pelleted Ca2+ was greatly diminished under these conditions, whereas the solubilized CaZ+ actually increases. This clearly indicates that the major proportion of the synaptosomal CaZ+ is located in a compar...

Introduction: Compounds which are used to treat organophosphate (OP) poisoning are not able to fully alleviate long lasting effects. They are mainly used to antagonize cholinergic effects of Ops. However, non-cholinergic effects, such as interference with different neurotransmitter systems, especially GABA release and uptake, are recently attracting more attentions. We have tried to investi...

Background: Neurotransmitter release is an essential link in cell communication of the nervous system. Many investigations have focused on gamma amino butyric acid (GABA)-ergic neurotransmission, because it has been implicated in the pathophysiology of several central nervous system disorders. To bypass complications related to homo- and heterosynaptic modulation and to avoid indirect interpret...