Aim: The purpose of this in vitro study was to evaluate if the marginal discrepancy and internal fit maxillary complex provisional bridge is improved by either 3D printing or CAD/CAM fabrication technique Methodology: In present a total sixteen samples (Eight each group) fabricated two methods using same STL file. Eight were from Telio CAD Subtractive CAM method (ARUM 5X- 400 Milling machine) (...

Sensory pathways process behaviorally relevant signals in various contexts and therefore have to adapt to differing background conditions. Depending on changes in signal statistics, this adjustment might be a combination of two fundamental computational operations: subtractive adaptation shifting a neuron's threshold and divisive gain control scaling its sensitivity. The cricket auditory system...

To identify genes associated with morphological phenotypes of human bladder transitional cell carcinoma, we used suppression subtractive hybridization (SSH) to create a subtractive cDNA library of two established cell lines, BLZ-211 and BLS-211, derived from a patient with transitional cell carcinoma of the bladder, then to screen for differentially expressed genes. Real-time reverse transcript...

This article presents a study of academic performance evaluation using soft computing techniques inspired by the successful application of K-means, fuzzy C-means (FCM), subtractive clustering (SC), hybrid subtractive clustering-fuzzy C-means (SC-FCM) and hybrid subtractive clustering-adaptive neuro fuzzy inference system (SC-ANFIS) methods for solving academic performance evaluation problems. M...

PURPOSE To evaluate the fit of a three-unit metal framework of fixed dental prostheses made by subtractive and additive manufacturing. MATERIALS AND METHODS One master model of metal was fabricated. Twenty silicone impressions were made on the master die, working die of 10 poured with Type 4 stone, and working die of 10 made of scannable stone. Ten three-unit wax frameworks were fabricated by...

BACKGROUND AND PURPOSE The purpose of this study is to identify new molecules that play important roles in the phenomena that occur in the hippocampus after transient global cerebral ischemia, as clues to better understanding of the mechanisms. METHODS A subtractive cDNA library was established by suppression subtractive hybridization of rat hippocampal tissues after transient global cerebral...

A surface plasmon resonance (SPR) immunosensor was developed for the detection of E. coli O157:H7 by means of a new subtractive inhibition assay. In the subtractive inhibition assay, E. coli O157:H7 cells and goat polyclonal antibodies for E. coli O157:H7 were incubated for a short of time, and then the E. coli O157:H7 cells which bound antibodies were removed by a stepwise centrifugation proce...

A genomics approach was used to identify nutritionally regulated genes involved in growth of fast skeletal muscle in Atlantic salmon (Salmo salar L.). Forward and reverse subtractive cDNA libraries were prepared comparing fish with zero growth rates to fish growing rapidly. We produced 7,420 ESTs and assembled them into nonredundant clusters prior to annotation. Contigs representing 40 potentia...

Speaker identification is the computing task to identify an unknown identity based on the voice. A good speaker identification system must have a high accuracy rate to avoid invalid identity. Despite of last few decades’ efforts, accuracy rate in speaker identification is still low. In this paper, we propose a hybrid approach of unsupervised and supervised learning i.e. subtractive clustering a...

A precise predictive model is important for obtaining a clear understanding of the changes in dissolved oxygen content in crab ponds. Highly accurate interval forecasting of dissolved oxygen content is fundamental to reduce risk, and three-dimensional prediction can provide more accurate results and overall guidance. In this study, a hybrid three-dimensional (3D) dissolved oxygen content predic...