Various strategies employed in genomic DNA cloning by subtractive hybridization have been examined by computer simulations, with the comparison between the predictions and the published results. The result shows that the efficiency of target sequence enrichment and the sensitivity to experimental conditions depend strongly on the enrichment strategy employed. The strategy selecting only tester/...

The effect of different kinds of weight normalization on the outcome of a simple competitive learning rule is analysed. It is shown that there are important differences in the representation formed depending on whether the constraint is enforced by dividing each weight by the same amount (“divisive enforcement”), or subtracting a fixed amount from each weight (“subtractive enforcement”). For th...

Prediction of cutting forces is very important for the design of cutting tools and for process planning. This paper presents a fuzzy modelling method of cutting forces based on subtractive clustering. The subtractive clustering combined with the least-square algorithm identifies the fuzzy prediction model directly from the information obtained from the sensors. In the micro-milling experimental...

: Answering a question by Honsell and Plotkin, we show that there are two equations between λ-terms, the so-called subtractive equations, consistent with λ-calculus but not simultaneously satisfied in any partially ordered model with bottom element. We also relate the subtractive equations to the open problem of the order-incompleteness of λ-calculus, by studying the connection between the noti...

This paper investigates mechanisms underlying the coining of intentional morphological blends and complex clippings. In one case study, I investigate the degree to which (a corpus-based definition of) psycholinguistic recognition points play a role in these subtractive word-formation processes. Also, I am concerned with the issue whether a separation of these two categories, which has been embr...

We extend the subtractive imaging method to label-free second harmonic generation (SHG) microscopy to enhance the spatial resolution and contrast. This method is based on the intensity difference between two images obtained with circularly polarized Gaussian and doughnut-shaped beams, respectively. By characterizing the intensity and polarization distributions of the two focused beams, we verif...

This series of studies represents an effort to extend the subtractive method of Bonders to the analysis of depth of processing in simple classification tasks. The stimuli are always pairs of items (letters, nonsense forms, digits) to which S must respond "same" or "different" as quickly as possible. Levels of instruction are physical identity (e.g., AA), name identity (e.g., Aa), and rule ident...

In this paper we propose a new technique, wobbling, for the stabilization of spectral ADC-test parameters with respect to offset and amplitude deviations of the sinusoidal stimulus. Wobbling aims at removing the effect of the rounding operation that takes place in an ADC, so that the measured harmonic distortion and noise amplitude can be truly ascribed to the intrinsic non-linearity and noise ...