The function of subcellular structures is defined by their specific sets of proteins, making subcellular protein localization one of the most important topics in organelle research. To date, many organelle proteomics workflows involve the (partial) purification of the desired subcellular structure and the subsequent analysis of the proteome using tandem mass spectrometry (MS/MS). This chapter g...

1. Incubation conditions were established in experiments with human-prostate homogenates for almost complete conversion of [4-(14)C]testosterone into at least ten transformation products. 2. Whole homogenates of tissue with benign hypertrophy were shown to contain 3alpha-, 3beta- and 17beta-hydroxy steroid dehydrogenases, Delta(4)-3-oxo steroid 5alpha- and 5beta- reductases and unidentified hyd...

Peritoneal leukocytes harvested from rabbits which have received two spaced doses of endotoxin have significantly greater (10-fold) coagulant activity than leukocytes from control rabbits. The coagulant activity accelerates the clotting of normal plasma and activates factor X in the presence of factor VII and calcium and is therefore regarded as tissue factor. A total of 40-80 mg tissue factor ...

Natural antisense transcripts and overlapping sense transcripts are expressed in a variety of tissues, including adult mouse brain. Here we show that a subset of mRNA-like sense-antisense transcript pairs are co-expressed within synaptoneurosomes of adult mouse forebrain, a subcellular fraction that is enriched in pinched-off dendritic spines of pyramidal neurons. Several of these pairs involve...

The intra- and transcellular transports of hepatic secretory and membrane proteins were studied in rats in vivo using [3H]fucose and [35S]cysteine as metabolic precursors. Incorporated radioactivity in plasma, bile, and liver subcellular fractions was measured and the labeled proteins of the Golgi complex, bile, and plasma were separated by SDS PAGE and identified by fluorography. 3H-radioactiv...

The two tripeptides are similar in structure; they share some biological properties (Cliffe & Waley, 1958b), and compete in others (Cliffe & Waley, 1959a) and are synthesized by the same route in extracts of calf lens (Cliffe & Waley, 1958a). The comparison between ophthalmic acid and glutathione has now been extended by the choice of a tissue that not only contains enzymes that bring about the...

Using monoclonal antibodies we have characterized a brain protein that copurifies with microtubules. We identify it as a microtubule-associated protein (MAP) by the following criteria: it copolymerizes with tubulin through repeated cycles of microtubule assembly in vitro; it is not associated with any brain subcellular fraction other than microtubules; in double-label immunofluorescence experim...

Two independent experiments indicated that the major subcellular site of de novo dolichyl phosphate biosynthesis in rat liver is in the microsomes. First, when purified subcellular fractions were assayed for long chain prenyltransferase activity, the profile obtained indicated exclusively a microsomal localization. Second, when liver slices were incubated 60 min with [14C]acetate and subcellula...

The differences in percentage inhibition by hexafluorenium bromide [hexamethylene1,6-bis-(9-fluorenyldimethylammonium) dibromide (Mylaxa, Nessler, Mallinckrodt, Germany)] and in polyacrylamide-gel disc-electrophoretic pattern of the hydrolytic activity of acetylthiocholine iodide of different fractions of rat jejunum have been reported by Das & Sayer (1 975). Scaf (1971) in his study on the eff...

Enzyme activities involved in glucose utilization via the Embden-Meyerhof and the pentole phosphate pathways were compared in subcellular fractions prepared from: (a) normal mammary glands excised from virgin, pregnant, lactating, and postlactating C3H mice; (6) HAN* outgrowths from virgin mice; (c) spontaneous mammary adenocarcinomas from virgin, pregnant, and lactating mice; and (d) mammary a...