PURPOSE In early exudative age-related macular degeneration (AMD), segmental thinning of Bruch's membrane is associated with ingrowth of choroidal neovascularization into the subretinal space. To determine whether there is a link between oxidative stress and extracellular matrix (ECM) degradation by the retinal pigment epithelium, the present study focused on the effect of oxidative stress on M...

AIMS Phosphorylation of forkhead box O (FoxO) transcription factors induces their nuclear exclusion and proteosomal degradation. Here, we investigated the effect of fluid shear stress on FoxO1a in primary cultures of human endothelial cells and the kinases that regulate its phosphorylation. METHODS AND RESULTS Shear stress (12 dynes/cm2) elicited the phosphorylation, nuclear exclusion, and de...

When optimizing an accelerated degradation testing (ADT) plan, the initial values of unknown model parameters must be pre-specified. However, it is usually difficult to obtain the exact values, since many uncertainties are embedded in these parameters. Bayesian ADT optimal design was presented to address this problem by using prior distributions to capture these uncertainties. Nevertheless, whe...

Bacterial toxin-antitoxin (TA) systems are genetic elements composed of a toxin gene and its cognate antitoxin, with the ability to regulate growth. TA systems have not previously been reported in marine Synechococcus or Prochlorococcus. Here we report the finding of seven TA system pairs (Type II) in the estuarine Synechococcus CB0101, and their responses of these TA genes to under different s...

Cells respond to stress by remodeling their transcriptome through transcription and degradation. Xrn1p-dependent degradation in P-bodies is the most prevalent decay pathway, yet, P-bodies may facilitate not only decay, but also act as a storage compartment. However, which and how mRNAs are selected into different degradation pathways and what determines the fate of any given mRNA in P-bodies re...

A correlation between the gated-diode R-G current and the performance degradation of SOI n-channel MOS transistor after F-N stress test has been demonstrated in this paper. Due to increase of interface traps after F-N stress test, the generation-recombination (R-G) current of the gateddiode in the SOI-MOSFET architecture increases while the performance characteristics of MOSFET transistor such ...

The ability to withstand mitochondrial damage is especially critical for the survival of postmitotic cells, such as neurons. Likewise, cancer cells can also survive mitochondrial stress. We found that cytochrome c (Cyt c), which induces apoptosis upon its release from damaged mitochondria, is targeted for proteasome-mediated degradation in mouse neurons, cardiomyocytes, and myotubes and in huma...

Abstract: This paper deals with the design of Optimal Step-Stress Partially Accelerated Degradation Test (PADT) using Wiener Process for modelling degradation paths. In partial step-stress loading, the unit is tested at usual stress for some time and then stress is accelerated. This helps in preventing over-stressing the test specimens. Failure occurs when the performance characteristic crosses...

Gate degradation in high electron mobility transistors (HEMTs) under OFF-state stress results from the high electric field near the gate edge. We investigate the evolution of this field over time in AlGaN/ GaN HEMTs upon OFF-state stress using a combination of electroluminescence (EL) microscopy and spectroscopy. EL analysis suggests that the electric field at the sites of generated surface def...

a simple and rapid stability indicating method based on capillary zone electrophoresis has been developed and validated for the analysis of buserelin (bus). the best separations were achieved by using a bare fused silica capillary (75 m i.d.; 65.5 cm total, 57.0 cm effective length), phosphate buffer (ph = 3.00; 26.4 mm), at 35 °c. the sample was hydrodynamically injected at 50 mbar for 5 seco...