نتایج جستجو برای: stranded rna dsrna

تعداد نتایج: 273179  

Journal: :Journal of immunology 2008
Behnam Naderi Kalali Gabriele Köllisch Jörg Mages Thomas Müller Stefan Bauer Hermann Wagner Johannes Ring Roland Lang Martin Mempel Markus Ollert

Emerging evidence suggests an important role for human epidermal keratinocytes in innate immune mechanisms against bacterial and viral skin infections. The proinflammatory effect of viral infections can be mimicked by double-stranded RNA (dsRNA). Herein, we demonstrate that keratinocytes express all known dsRNA sensing receptors at a constitutive and inducible level, and that they use several d...

Journal: :Molecular cell 2008
Tetsushi Iida Jun-ichi Nakayama Danesh Moazed

Heterochromatic gene silencing at the pericentromeric DNA repeats in fission yeast requires the RNA interference (RNAi) machinery. The RNA-induced transcriptional silencing (RITS) complex mediates histone H3 lysine 9 (H3K9) methylation and recruits the RNA-dependent RNA polymerase complex (RDRC) to promote double-stranded RNA (dsRNA) synthesis and siRNA generation. Here we show that ectopic exp...

Abolghasem Ajami, Alireza Rafiei, Eshrat Gharaee, Fatemeh Rahbarizadeh, Mohammad bagher Hashemi Sooteh, Mohammad Soltany-Rezaee-Rad, Negar Mottaghi-Dastjerdi, Saeid Abediankenari,

Background: RNAi (RNA interference) is a new strategy in gene therapy and biotechnology which provides new promises in the treatment of different diseases such as cancer and viral diseases. CCND1 which is a key gene in cell cycle is amplified and over expressed in esophageal cancer. The objective of this study was production and siRNAs for CCND1, the key gene in cell cycle. Materials and Metho...

2012
Yu-liang Zhang Shu-zhen Zhang Mahesh Kulye Su-ran Wu Nai-tong Yu Jian-hua Wang Hong-mei Zeng Zhi-xin Liu

RNA interference (RNAi) is a technology for conducting functional genomic studies and a potential tool for crop protection against insect pests. Development of reliable methods for production and delivery of double-stranded RNA (dsRNA) is the major challenge for efficient pest control. In this study, Chilo infuscatellus Snellen (Crambidae: Lepidoptera) was fed with CiHR3 dsRNA expressed in bact...

Journal: :Journal of molecular biology 2008
Greg S Parker Tuhin Subhra Maity Brenda L Bass

Double-stranded RNA (dsRNA)-binding proteins facilitate Dicer functions in RNA interference. Caenorhabditis elegans RDE-4 facilitates cleavage of long dsRNA to small interfering RNA (siRNA), while human trans-activation response RNA-binding protein (TRBP) functions downstream to pass siRNA to the RNA-induced silencing complex. We show that these distinct in vivo roles are reflected in in vitro ...

Journal: :Journal of virology 2004
Bradley I Hillman S Supyani Hideki Kondo Nobuhiro Suzuki

RNA viruses of filamentous fungi fall into two broad categories, those that contain double-stranded RNA (dsRNA) genomes in rigid particles and those that are more closely related to positive-sense, single-stranded RNA viruses with dsRNA replicative intermediates found within lipid vesicles. Effective infectivity systems have been described for the latter, using RNA transcripts, but not for the ...

2014
Ji-Seung Yoo Kiyohiro Takahasi Chen Seng Ng Ryota Ouda Koji Onomoto Mitsutoshi Yoneyama Janice Ching Lai Simon Lattmann Yoshikuni Nagamine Tadashi Matsui Kuniyoshi Iwabuchi Hiroki Kato Takashi Fujita

RIG-I is a DExD/H-box RNA helicase and functions as a critical cytoplasmic sensor for RNA viruses to initiate antiviral interferon (IFN) responses. Here we demonstrate that another DExD/H-box RNA helicase DHX36 is a key molecule for RIG-I signaling by regulating double-stranded RNA (dsRNA)-dependent protein kinase (PKR) activation, which has been shown to be essential for the formation of antiv...

Journal: :FEBS Open Bio 2023

Y‐satellite RNA (Y‐sat) of cucumber mosaic virus upregulates the expression aphid ABCG4 gene, which promotes wing formation. We used virus‐induced gene silencing (VIGS) to prevent wing‐induction mechanism Y‐sat and thus inhibited Of aphids on plants with VIGS , only about 30% had wings, 60–70% winged were small likely impaired in flying ability. In addition, we showed that double‐stranded RNAs ...

Journal: :Phytopathology 2011
Xiuchun Zhang Shirley Sato Xiaohong Ye Anne E Dorrance T Jack Morris Thomas E Clemente Feng Qu

Transgenic plants expressing double-stranded RNA (dsRNA) of virus origin have been previously shown to confer resistance to virus infections through the highly conserved RNA-targeting process termed RNA silencing or RNA interference (RNAi). In this study we applied this strategy to soybean plants and achieved robust resistance to multiple viruses with a single dsRNA-expressing transgene. Unlike...

2015
Justine M. Pompey Bardees Foda Upinder Singh Xi Zhou

Dicer enzymes process double-stranded RNA (dsRNA) into small RNAs that target gene silencing through the RNA interference (RNAi) pathway. Dicer enzymes are complex, multi-domain RNaseIII proteins, however structural minimalism of this protein has recently emerged in parasitic and fungal systems. The most minimal Dicer, Saccharomyces castellii Dicer1, has a single RNaseIII domain and two double ...

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