نتایج جستجو برای: stimulated by his environment

تعداد نتایج: 7350401  

2016
Alia A. Sagatova Mikhail V. Keniya Rajni K. Wilson Manya Sabherwal Joel D. A. Tyndall Brian C. Monk

Emergence of fungal strains showing resistance to triazole drugs can make treatment of fungal disease problematic. Triazole resistance can arise due to single mutations in the drug target lanosterol 14α-demethylase (Erg11p/CYP51). We have determined how commonly occurring single site mutations in pathogenic fungi affect triazole binding using Saccharomyces cerevisiae Erg11p (ScErg11p) as a targ...

Journal: :Cancer research 2004
Christina L van Broekhoven Christopher R Parish Caroline Demangel Warwick J Britton Joseph G Altin

Dendritic cells (DCs) are potent stimulators of immunity, and DCs pulsed with tumor antigen ex vivo have applications in tumor immunotherapy. However, DCs are a small population of cells, and their isolation and pulsing with antigen can be impractical. Here we show that a crude preparation of plasma membrane vesicles (PMV) from the highly metastatic murine melanoma (B16-OVA) and a surrogate tum...

Journal: :Biochemistry 2003
Tanja Gerharz Stefan Reinelt Sibylle Kaspar Leonardo Scapozza Michael Bott

The sensor kinase CitA and the response regulator CitB of Klebsiella pneumoniae form the paradigm of a subfamily of bacterial two-component regulatory systems that are capable of sensing tri- or dicarboxylates in the environment and then induce transporters for the uptake of these compounds. We recently showed that the separated periplasmic domain of CitA, termed CitAP (encompasses residues 45-...

Journal: :Methods in molecular biology 2011
Ping Sun Joseph E Tropea David S Waugh

In the field of biotechnology, fusing recombinant proteins to highly soluble partners is a common practice for overcoming aggregation in Escherichia coli. E. coli maltose-binding protein (MBP) has been recognized as one of the most effective solubilizing agents, having frequently been observed to improve the yield, enhance the solubility, and promote the proper folding of its fusion partners. T...

Journal: :Methods in molecular biology 2007
Joseph E Tropea Scott Cherry Sreedevi Nallamsetty Christophe Bignon David S Waugh

A generic protocol that utilizes a dual hexahistidine-maltose-binding protein (His6-MBP) affinity tag has been developed for the production of recombinant proteins in Escherichia coli. The MBP moiety improves the yield and enhances the solubility of the passenger protein while the His-tag facilitates its purification. The fusion protein (His6-MBP-passenger) is purified by immobilized metal affi...

2012
Amy Stroud Susan Liddell Thorsten Allers

Single-stranded DNA (ssDNA)-binding proteins play an essential role in DNA replication and repair. They use oligonucleotide/oligosaccharide-binding (OB)-folds, a five-stranded β-sheet coiled into a closed barrel, to bind to ssDNA thereby protecting and stabilizing the DNA. In eukaryotes the ssDNA-binding protein (SSB) is known as replication protein A (RPA) and consists of three distinct subuni...

Journal: :Infection and immunity 2001
J Oetjen P Fives-Taylor E Froeliger

A gene encoding an endopeptidase from Streptococcus parasanguis FW213 has been cloned and shown to have high sequence homology to genes encoding mammalian metalloendopeptidases. The gene, designated S. parasanguis pepO, was cloned into the pET28a expression vector, resulting in a fusion of vector sequences encoding a hexahistidine tag at the carboxyl terminus. The recombinant PepO (rPepO) was e...

Journal: :Chembiochem : a European journal of chemical biology 2002
Ziye Liu Jianbo Zhang Xi Chen Peng G Wang

Regeneration of sugar nucleotides is a critical step in the biosynthetic pathway for the formation of oligosaccharides. To alleviate the difficulties in the production of sugar nucleotides, we have developed a method to produce uridine diphosphate galactose (UDP-galactose). The combined biosynthetic pathway, which involves seven enzymes, is composed of three parts: i) the main pathway to form U...

Journal: :Biochemistry 2007
Walter De Laurentis Leang Khim J L Ross Anderson Ariane Adam Kenneth A Johnson Robert S Phillips Stephen K Chapman Karl-Heinz van Pee James H Naismith

Pyrrolnitrin is a commonly used and clinically effective treatment for fungal infections and provides the structural basis for the more widely used fludioxinil. The pyrrolnitrin biosynthetic pathway consists of four chemical steps, the second of which is the rearrangement of 7-chloro-tryptophan by the enzyme PrnB, a reaction that is so far unprecedented in biochemistry. When expressed in Pseudo...

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