Little did Christian Gram1 know that his differential staining technique developed in 1884 would remain one of the first procedures learned by beginning bacteriology students and one of the first procedures carried out in any laboratory where bacteria are routinely identified at the end of the 20th century. In today's high-tech medical world where the utilization of nucleic-acid amplification i...

Several studies have already described the presence of specialized niches of precursor cells in vasculature wall, and it has been shown that these populations share several features with mesenchymal stromal cells (MSCs). Considering the relevance of MSCs in the cardiovascular physiopathology and regenerative medicine, and the usefulness of the pig animal model in this field, we reported a new m...

INTRODUCTION Campylobacter species are recognized as a major cause of acute gastroenteritis in humans throughout the world. The diagnosis is mainly based on stool culture. This study was done to evaluate the effectiveness of staining methods (Gram stain using 0.3% carbol fuchsin as counter stain and 1% carbol fuchsin direct stain) versus culture as the gold standard. METHODS A total of 300 ch...

In recent years, with the rapid development of building industry in western cities during period “Western Development” China, paints has developed rapidly and become more mature. addition, waterborne inorganic exterior wall have been a great choice because their reduced volatile organic compounds (VOCs) less toxicity odor. However, problem stain resistance for not solved, which major obstacle t...

An evaluation of the standardized Romanowsky stain of Marshall et al. has been made in a routine haematology laboratory. It was noted that this stain had several advantages over the May-GrUnwald Giemsa stain used in most British laboratories. These advantages include ease and speed of preparation, a shorter staining time, and reproducibility of results. These results are described in detail. Th...

In our study of the histogenesis of the miliary tubercle developing inside the liver lobule in animals that have been stained vitally while inoculated with bovine tuberculosis, the controls enable us to recognize the manner in which the vital stain affects the liver. There is therefore no possibility of confusing the effects due to the organism with the effects due to the dye. It is, however, o...

Experimental.-The suspension of tuberde bacilli used was prepared by grinding a loopful (about 3 mg.) of bovine tuberde bacilli with sterile mortar and pestle and suspending them in I cc. of sterile, normal saline. The final series of observations (after exdusion of cases complicated by thrombus formation or obvious errors in technic) comprises 15 animals, autopsied at intervals of 6, 12, 24, a...

Zygotes of the brown alga Fucus distichus L. Powell accumulate a sulfated polysaccharide (fucoidin) in the cell wall at the site of rhizoid formation. Previous work indicated that zygotes grown in seawater minus sulfate do not sulfate the preformed fucan (an unsulfated fucoidin) but form rhizoids. Under these conditions, we determined whether sulfation of the fucan is required for its localizat...