INTRODUCTION Helicobacter pylori is an important pathogen in humans causing chronic gastritis & playing a major role in the development of peptic ulcers & gastric cancer [1]. The present study was undertaken to find out the presence of H.pylori in various gastroduodenal diseases & to evaluate the advantages of Loeffler’s methylene blue (LMB) stain for crushed biopsy smears. The study was conduc...

A new and convenient myelin stain is described. Paraformaldehyde fixed tissue is serially immersed in a nitroblue tetrazolium solution and then in a diaminobenzidine solution. The result is distinct blue staining of myelinated fiber tracts. This technique has advantages over presently used myelin stains.

1. Rat liver mitochondria were examined in the electron microscope by using negative staining in the presence of 0.3m-sucrose. The intact outer membrane does not appear to be freely permeable to the stain. Where the stain penetrated through a tear it was seen that the inner membrane had randomly oriented grooves, many of which contained round structures varying between 200 and 900å in diameter....

The knowledge of vascular structures of port wine stains (PWSs) may be useful to select treatment doses and improve therapeutic efficacy. Biopsies are impractical to implement, therefore, it is necessary to develop non-invasive techniques for morphological evaluation. This study aimed to evaluate the application of a novel optical coherence tomography (OCT) system to characterize the vascular s...

How well an organism completes an ecologically relevant task - its performance - is often considered a key factor in determining individual fitness. Historically, ecomorphological studies have examined how morphological traits determine individual performance in a static manner, assuming that differential fitness in a population is due indirectly to differences in morphological traits that dete...

1. The nurse cells of Rhodnius possess nucleoli that stain with Heidenhain's hematoxylin but give a negative Feulgen reaction. In localized positions adjacent to the nuclear membrane are seen masses of material both within the nucleus and the adjoining cytoplasm that stain with Heidenhain's hematoxylin, but, like the nucleolus, give a negative Feulgen reaction. 2. Electron micrographs of the nu...

Background: Prior research demonstrated that via a novel technique it was possible to obtain ex vivo hair tissues magnetic profiles based on microscopic nano-sized iron particles detected by Prussian Blue Stain (PBS). The present study demonstrates that magnetic signals emitted by both the ex vivo and in vivo hair follicles could penetrate glass barriers. Methods: Two different versions of Prus...

PURPOSE To evaluate the efficacy and safety of two oscillating/rotating toothbrushes, (Braun Oral-B Plaque Remover-D7 and Braun Oral-B Ultra Plaque Remover-D9) and a manual toothbrush with respect to removing extrinsic dental stain. MATERIALS AND METHODS This randomized, three-way cross-over study investigated 24 subjects entering the trial, which was divided into three, 5-day periods. Over t...

X-ray microscopy (XRM) is the only microscopy technique that can provide high-resolution (30 nm) imaging of biological specimens without the need to fix, stain or section them. We aim to determine the effect, if any, of glutaraldehyde fixation on algae cells from the XRM perspective and thus provide beneficial information for both X-ray and electron microscopists on artefacts induced by glutara...

Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes [1]. Hematoxylin and eosin stain (H&E stain or HE stain), is the routine staining for skin biopsies. However, in some circumstances, d...