Starting from an alignment of all known representatives in GenBank, we designed group specific primers targeting SSU rRNA-encoding sequences of 12 microbial taxa known to contain insect pathogens and symbionts. We tested the specificity of these primers using representative species of all 12 groups as control templates. Polymerase chain reaction amplification conditions were modified until only...

Zygotylenchus gansuensis n. sp. is described and illustrated from the rhizosphere of jujube (Zizyphus jujuba Mill.) based on morphology and molecular analyses. This new species is characterized by a low and flattened labial region with three annuli, stylet 14.1±0.5 (13.0-14.9) μm long, deirids absent, five lateral lines in the vulval region, pharyngeal glands overlapping ventrally or ventrolate...

Three trachelocercid ciliates, Kovalevaia sulcata (Kovaleva, 1966) Foissner, 1997, Trachelocerca sagitta (Müller, 1786) Ehrenberg, 1840 and Trachelocerca ditis (Wright, 1982) Foissner, 1996, isolated from two coastal habitats at Qingdao, China, were investigated using live observation and silver impregnation methods. Data on their infraciliature and morphology are supplied. The small subunit rR...

Molecular tools have revolutionized the exploration of biodiversity, especially in organisms for which traditional taxonomy is difficult (Tang et al., 2012). The small subunit (SSU) 18S rRNA gene is one of the most frequently used genes in phylogenetic studies and an important marker for random target PCR in environmental biodiversity screening. 18S rDNA is a nuclear ribosomal gene, commonly re...

Theileria buffeli, generally a benign parasite of cattle, has been reported in animals from Texas, Missouri and North Carolina. To date, there have been no reports of the parasite in cattle residing in northern portions of the US. An 8-year-old cow (Maine Anjou x Angus cross-bred) in Michigan presented with hemoglobinuria and a packed cell volume of 9. Blood films stained with Giemsa showed num...

The European database of the Small Subunit (SSU) Ribosomal RNA is a curated database that strives to collect all information about the primary and secondary structure of completely or nearly-completely sequenced rRNAs. Furthermore, the database compiles additional information such as literature references and taxonomic status of the organism the sequence was derived from. The database can be co...

giardia duodenalis is globally recognized as an important zoonotic intestinal protozoan parasite. so far, eight assemblages of g. duodenalis (a-h) have been identified. substantial evidence suggests the zoonotic potential of assemblages a, b, and e in livestock. in this study, the genotype of giardia duodenalis isolates was genetically identified by determining the sequence of ssu-rrna gene and...

SUMMARY MIAH is a WWW server for the automatic alignment of new eukaryotic SSU rRNA sequences to an existing alignment of 1500 sequences. AVAILABILITY http://chah.ucc.ie/MIAH Contact :