The generation of plaque-forming cells (PFC) to T-dependent antigen, but not to T-independent antigen, is reduced in vitro by Lyb-2 antibody. Monoclonal Lyb-2 antibody, added to Mishell-Dutton cultures within the first 2 d, but not later, greatly reduces the generation of alpha-sheep erythrocyte (SRBC) PFC from T-depleted spleen cells whether help is provided in the form of intact T cells or as...

The kinetics of the in vivo response to SRBC was studied in mouse spleen at both the B cell and T cell levels. The B cell response was assayed by following the appearance of antibody-secreting cells in the spleen using the hemolytic plaque assay. The T cell response was monitored by following the increase in or "priming" of helper activity in the spleen using a quantitative in vitro assay. The ...

Betulinic acid is a pentacyclic triterpene found in many plant species, among others, in the bark of white birch Betula alba. Betulinic acid was reported to display a wide range of biological effects, including antiviral, antiparasitic, antibacterial, anticancer and anti-inflammatory activities. The effects of betulinic acid (50, 5, 0.5 mg/kg) administered orally five times at 24 hours interval...

The objective of this study was to evaluate the effects of cottonseed meal (CSM) treated with sodium bentonite (SB) on certain blood components and immune response of laying hens. A 3 × 3 factorial arrangement with 3 levels of SB (0, 10, and 20 g/kg of diet) and 3 levels of CSM (0, 100 and 200 g/kg of diet) were used within 9 dietary treatments of 4 replicates each. Nine mash diets were fed to ...

s of papers 3 7 Non-MHC alloantigen genes affecting immunity. W. E. Briles*, Northern Illinois University, DeKalb, IL 60115. An alloantigen is a genetically determined cell-surface molecule detected by specific antisera. An identifying letter has been assigned to each genetic locus responsible for the eleven distinct families of alloantigens A, C, D, E, H, I, J, K, L, P, and R. The genes of eac...

An experimental system was developed to analyse quantitatively specific B cells for various antigens in human B cell populations by polyclonal transformation and immunoglobulin production induced by in vitro infection with Epstein-Barr virus (EBV). EBV-infected cells were cultured in microtest plates for three weeks and specific antibody activities for sheep red blood cell (SRBC) were detected ...

In cirrhosis, the phagocytic function of the reticuloendothelial system (RES) is decreased. In order to investigate the mechanisms of the hepatic reduced phagocytic activity present in cirrhosis, the hepatic and splenic uptake of 51Cr sheep red blood cells (SRBC) and of colloidal carbon was measured in three groups of Sprague-Dawley rats. Group 1 consisted of 42 control rats, group 2 of 36 rats...

Treatment of alligator serum with different concentrations of EDTA resulted in a concentration-dependent inhibition of serum-mediated sheep red blood cell (SRBC) hemolysis. This inhibition of serum-dependent hemolysis was observed for other chelators of divalent metal ions, such as phosphate and citrate. Treatment of alligator serum with 5 mM EDTA completely inhibited SRBC hemolysis, which coul...

In the present study, a selection experiment for antibody (Ab) titer against SRBC in chickens was analyzed. Two lines were divergently selected for increased and decreased Ab titer. Further, a randombred control line, originating from the same base population, was included in the experiment. The heritability for immune response against SRBC was estimated after 18 generations of selection. In to...

The media and culture conditions required for in vitro stimulation of mouse lymphoid cells are described. The medium was arginine-rich and contained heat-inactivated human serum. A component of the human sera necessary for stimulation of the cells was a natural mouse cell agglutinin, which affected both background stimulation and the degree of induced stimulation with phytohemagglutinin (PHA). ...