To compare the efficacy of 2 sperm-retrieval procedures, testicular sperm extraction (TESE) and testicular sperm aspiration (TESA), during the same procedure using the same subjects as their own controls. The presence of mature testicular sperm cells and motility were evaluated in 87 men with nonobstructive azoospermia (NOA) by means of multifocal TESE and multifocal TESA, which were performed ...

The aim of this study was to assess the predictive value of the hypoosmotic sperm swelling test. Good correlations were observed between the percentage of spermatozoa undergoing swelling and the percentage motility both in normal (r = 0.68; p < 0.001) and infertile patients (r = 0.65; p < 0.001). In contrast, sperm swelling did not significandy correlate either with sperm oocyte fusion (assesse...

In the female reproductive tract, mammalian sperm undergo a regulated sequence of prefusion changes that "prime" sperm for fertilization. Among the least understood of these complex processes are the molecular mechanisms that underlie sperm guidance by environmental chemical cues. A "hard-wired" Ca(2+) signaling strategy that orchestrates specific motility patterns according to given functional...

To assess the relative impact of functional constraint and post-mating sexual selection on sequence evolution of reproductive proteins, we examined 169 primate sperm proteins. In order to recognize potential genome-wide trends, we additionally analysed a sample of altogether 318 non-reproductive (brain and postsynaptic) proteins. Based on cDNAs of eight primate species (Anthropoidea), we observ...

Since all domestic turkeys are produced through artificial insemination, a measurable sperm characteristic that would be predictive of fertility would allow for the culling of poor males, resulting in improved reproductive efficiency. The sperm mobility test (SMT), which quantifies sperm penetration into an Accudenz solution, has been shown to correlate highly with fertilization potential of in...

OBJECTIVE To determine whether peritoneal fluid from women with endometriosis contributes to infertility by impairing sperm motion and functional characteristics. METHODS Women with endometriosis (n = 20) underwent laparoscopy for infertility or pelvic pain. Patients undergoing tubal ligation served as controls (n = 14). Peritoneal fluid was aspirated from women with endometriosis, or from wo...

In the present paper, morphological and functional features of human sperm midpiece, contributing to the assessment of sperm fertility potential, have been described. The NADH-dependent NBT screening assay was used to identify and visualise: 1/ morphological defects of sperm midpiece, 2/ immature sperm forms with extensive cytoplasmic retention, reflecting developmental failure in spermatogenic...

Genetic correlations between production traits (average daily gain from birth till the end of the field test and ultrasonically predicted lean meat content at the end of the field test) and semen traits (semen volume, sperm concentration, motility, percentage of abnormal spermatozoa, total number of spermatozoa and number of functional spermatozoa) were estimated from a large dataset (44 500 ob...

Semen analysis (SA) is the cornerstone for male infertility evaluation. Variations in sperm quantity and quality may make SA an unreliable decision-making tool in providing an insight of infertility (1). Consequently, efforts have been made to upgrade techniques of male fertility analysis. In recent times, primary investigative techniques have centered around assessing sperm capacity, sperm fun...