background: irradiation is one of the major causes of induced sperm dna damage. various studies suggested a relation between sperm dna damage and fertilization rate after intra-cytoplasmic sperm injection (icsi). objective: in this study, fertilization rate and premature chromosome condensation (pcc) formation after icsi of hamster oocytes with irradiated sperms from normal and oligosperm indiv...

OBJECTIVE The aim of this study was to evaluate the influence of different periods of abstinence on conventional semen parameters as well as functional parameters in human semen, including mitochondrial function, chromatin packing and sperm DNA fragmentation. METHODS We recruited a cohort of 2,458 men undergoing infertility investigation. Semen analyses were performed according to WHO guideli...

The ability to detect nuclear damage is an important tool for the development of sperm preservation methods. We used the acridine orange test (AOT) and the terminal deoxynucleotidyl transferase-mediated dUDP nick-end labeling (TUNEL) assay to assess the DNA status of sperm cells preserved with different lyophilization media. The AOT did not detect any differences between different lyophilizatio...

sperm is particularly susceptible to reactive oxygen species (ros) during critical phases of spermiogenesis. however, the level of seminal ros is restricted by seminal antioxidants which have beneficial effects on sperm parameters and developmental potentials. mitochondria and sperm plasma membrane are two major sites of ros generation in sperm cells. besides, leukocytes including polymer phonu...

Background: One of the major factors affecting on male infertility is oxidative stress (OS). Two main sources of ROS production are leukocytes and spermatozoa. The ROS such as tertiary-butyl hydroperoxide (TBHP) may damage and fragmentate to sperm DNA, which can lead to complications in the offspring. In this study, we will investigate the effect of od on DNA fragmentation index (DFI) in sperm ...

OBJECTIVE To determine DNA damage as measured by the sperm chromatin structure assay (SCSA) in subsets of human spermatozoa at different stages of maturation in patients who are undergoing infertility evaluation. DESIGN Prospective study. SETTING Andrology laboratory at a tertiary care hospital. PATIENT(S) Fifty-six patients undergoing infertility evaluation. Patients with normal semen pa...

cyclophosphamide (cp) is one of the anti-neoplastic drugs. despite its numerous clinical applications, it has devastating effects on the testicles and declines the sperm quality in treated patients. this study was aimed to investigate the protective effect of crocin in improving the toxicity induced by cp in reproductive system. in this study, 24 male adult mice (6 to 8 weeks) were randomly div...

Damage to the DNA of germ cells can lead to mutation, which may result in birth defects, genetic diseases, and cancer. The very high endogenous rate of oxidative DNA damage and the importance of dietary ascorbic acid (AA) in preventing this damage has prompted an examination of these factors in human sperm DNA. The oxidized nucleoside 8-hydroxy-2'-deoxyguanosine (8-oxo-7,8-dihydro-2'-deoxyguano...

Background: Fluoxetine (FLX) is an Antidepressant compound. FLX produces undesired side effects including anxiety, sleep disturbances, sexual dysfunction and gastrointestinal disturbances. The grape is a significant source of nutritional antioxidants, such as polyphenols, anthocyanins, flavonids as well as biologically active dietary components. Present study was performed to investigate the pr...