Building on previous research, which indicated that scintillation cells perform differently at different elevations (Burkhart, 2005; Eberline, 1989; George, l983), a pressure chamber has been designed which allows for the simulation of scintillation cell use at different "elevations." The pressure chamber has been constructed in such a way as to accommodate a large flexible bag that is filled w...

Formalin is widely used in industry and in medicine (as tissue fixative and disinfectant).It contains reactive molecules which have been known for its cytotoxic effects. To evaluate the effect of formalin exposure on the testicular tissue and sperm parameter from neonatal period through physical and sexual maturity, 28 male Wister rats were assigned into two equal test and control groups. The t...

OBJECTIVE The objective of this study was to develop a reproducible method of establishing the concentration of yeast cells per milliliter of solution. METHODS Three methods of determining the number of yeast cells in solution were compared: Neubauer's counting chamber, spectrophotometry, and nephelometry. RESULTS All three methods were comparable and reproducible. The following formulas we...

Background: One of the main laboratory tests for evaluation of infertility is semen analysis (SA). However, additional tests may be suggested for further diagnosis of male fertility potentials. The seminal fluid contains sperms, non-sperm cells, and various types of lipids and glucose. Objective: The objective of this cross-sectional study was to correlate the sperm parameters with concentratio...

By HARRY J. FITCH T HERE ARE t-iso standard nuethods for counting platelets, the direct and the indirect. For the direct nuethod the blood is accurately diluted with au anst-icoagulamut sohutiomu, usually in a hemocytomet-er pipet, and the platelets are enumsuei’ated ims a counting chanuber.1 For the indirect nuethod the blood is diluted amid a drop is placed on an ordinary microscope slide. It...

BACKGROUND The objectives of this study were (i) to evaluate whether the combined use of Syto 16 and 7-amino-actinomycin-D (7-AAD) allows the detection of sperm apoptosis and (ii) to describe a new multiparameter flow cytometric method to assess simultaneously sperm concentration (SC), viability and apoptosis as well as leukocyte concentration. METHODS Semen samples from 68 patients were eval...