Objective: Berberine is a natural compound isolated from plants with multiple pharmacological activities, like antioxidant and anti-inflammatory activities. The present study was to assess the effect of berberine with different concentration and at different time on sperm motility, viability and sperm membrane integrity of humans. Methods: Semen samples were obtained from 30 patients (22–30 yea...

At ejaculation, human sperm are considered to be mechanically trapped and become immotile in the semen coagulum by binding to semenogelins (Sgs) from the seminal vesicle and zinc ions from the prostate. However, the physiological combined roles of the protein and heavy metal on sperm motility are unknown. Here, we have first demonstrated that Sg I alone, which does not form the semen coagulum w...

objective: to evaluate the effect of honey supplemented to cryoprotectant medium on post-thaw sperm motility, concentration, morphology and agglutination. materials and methods: thirty semen samples were collected from 30 infertile patients. after assessment of semen analysis, semen samples were divided into 3 aliquots (0.7ml for each) and mixed with 1 ml of cryopreservation solution (g1, contr...

The present study was conducted to determine correlation of semen quality with libido in different breeds of bulls. Four mature cattle bulls from each of Friesian, Jersey, Achai, Cross (Friesian x Sahiwal) and Sahiwal in different age (1-3, 3-5 and above 5 years) were selected. For age grouping, data of the entire breed was pooled. Libido was measured in terms of time (seconds) taken for the en...

This study was undertaken to investigate whether there is relationship between seminal plasma transferrin and seminal parameters which included sperm count, motility and morphology. The study included 100 male subjects in the age group of 23-41 yrs including 7 proven fertility, 6 post-vasectomised and 87 subjects were of idiopathic infertility. Estimation of seminal plasma transferrin concentra...

Matrix metalloproteinase (MMP)-2 and MMP-9 are gelatinases that take part in several reproductive processes. The aim of this study was to measure levels MMP-2 fractionated stallion ejaculates, evaluate the association between these components semen quality, sperm longevity during cooled storage. Semen quality were assessed separately for sperm-rich fractions (HIGH), sperm-poor (LOW), whole ejac...

Male pales weevils, Hylobius pales (Herbst), were sexually immature upon emergence from their pupal cells. During the first 2 wk of adult life the concentration of sperm bundles in the testes increased, and a lumen containing a granular material began to form in the accessory glands. Most individuals had active sperm when 2 wk old. Four-week-old males had a large concentration of sperm bundles,...

A combination of flow cytometric sperm sorting of X and Y chromosome-bearing sperm (X and Y sperm) and computer-assisted sperm analysis (CASA) for measuring sperm motility allows assessment of motion parameters in the two populations. Bull sperm were separated into X and Y populations by flow cytometry following staining with the DNA-binding dye Hoechst 33,342. The motion parameters differed de...

Participation rates in epidemiologic studies on semen quality are generally very low, raising concerns as to the potential for selection bias. Since hormones both initiate and maintain spermatogenesis, they may serve as surrogates of semen quality in epidemiologic studies. For this reason, in the present study, we explored the influence and predictive ability of reproductive and thyroid hormone...