Background and Aims: Due to the paucity of studies, association between the morphology and function of sperm and recurrent miscarriage (RM) is not yet completely known. Increased reactive oxygen species and decreased antioxidant levels in men have been shown to be associated with RM. Recently it has been accepted that antioxidant therapy can approve sperm parameters. The goal of this study was ...

Current methods of analyzing sperm chromatin competency overlook the inner sperm compartment which is inaccessible to probes and reagents. By breaking the molecular protamine disulfide bridges, the DNA toroids are exposed to integrity analysis. The aim was to develop a simple nuclear toroid test and determine its association with fertilization, pregnancy, and miscarriage. The approach involved ...

BACKGROUND Intrauterine insemination (IUI) is one of the therapeutic approaches for infertility. The objective of this study was to evaluate DNA integrity and apoptosis role in success of IUI in both mild male and female factor infertility. METHODS PATIENTS WERE DIVIDED INTO TWO GROUPS: M (mild male factor; n = 29) and F (female factor; n = 31) undergoing single IUI. Ejaculates were analyzed ...

We evaluated the effect of varicocelectomy on semen parameters and levels of sperm DNA damage in infertile men. A total of 75 infertile men with varicocele and 40 fertile men (controls) were included in this study. Semen analysis and sperm DNA damage expressed as the DNA fragmentation index using acridine orange staining and chromatin condensation test by flow cytometry were assessed before and...

introduction: the aim of this study was to evaluate the effect of sperm chromatin anomalies on fertilization outcome post icsi. material and methods: therefore along with semen parameters, cma3 staining for protamine deficiency, aniline blue staining for excessive histones, sds for sperm chromatin stability and sds+edta for ability of sperm to undergo decondensation were carried out on 55 semen...

BACKGROUND There are few studies indicating the detrimental effects of ibuprofen on sperm fertility potential and DNA integrity. OBJECTIVE To determine the effects of Ibuprofen on sperm parameters, chromatin condensation and DNA integrity of mice. MATERIALS AND METHODS In this experimental study, 36 adult male mice with average weight 37 gr were divided into three groups, including control ...

Chromosomes in terminally differentiated mammalian spermatozoa are extensively condensed by protamines but a small proportion of histones remain. We examined the primary organization of somatic-type chromatin in lysolecithin-permeabilized human sperm nuclei and report that nucleosomes are closely packed with a periodicity of approximately 150 bp. Incubation of nuclei in the presence of exogenou...

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. One of the measurements of sperm quality that can be assessed with validated protocols is DNA fragmentation, the subject was profoundly dissected in the article by Agarwal et al. and that constitutes the backbone of this commentary (1). Authors quoted: “Over the past decade, Sperm DNA Fragmentation (SDF) measurements ...

Background: It is showed that methamphetamine has harmful effects on male reproductive system. The aim of this study was to investigate the effects of methamphetamine administration on testis weight, sperm count, sperm morphology, and chromatin status as well as sperm apoptotis followed by the cytochemical tests in a mouse model. Materials and Methods: Twenty four NMRI male mice were divided in...