A radioimmunoassay procedure has been developed for the direct measurement of methotrexate (MTX) in plasma, serum, cerebrospinal fluid, or urine samples. The assay is sensitive to levels of at least 100 pg of MTX and is highly specific for MTX in the presence of folinic acid (citrovorum factor), folic acid, tetrahydrogolic acid, and other folate analogs and known metabolites. Results from this ...

OBJECTIVES Cefixime (Cfx), is a semi-synthetic third-generation oral cephalosporin antibiotic that is prescribed for the treatment of susceptible infections. There are some procedures for the determination of Cfx in pharmaceutical formulations and biological samples. Herein a spectrofluorimetric method was proposed for Cfx determination based on the fluorescence quenching of terbium-danofloxaci...

Alkaline pH is one of the most important problems of our aquatic habitat. We used Stigonematalean native cyanobacterium Fischerella sp. FS 18 as our model strain, andstudied it under different alkaline pHs (7, 9 and rarely 11) under two different – short and long- time treatments (24 and 96 hours after inoculation). Spectroscopic results showed that both alkalinity and time affected growth rate...

Objective(s):Cefixime (Cfx), is a semi-synthetic third-generation oral cephalosporin antibiotic that is prescribed for the treatment of susceptible infections. There are some procedures for the determination of Cfx in pharmaceutical formulations and biological samples. Herein a spectrofluorimetric method was proposed for Cfx determination based on the fluorescence quenching of terbium-danofloxa...

Interaction of sanguinarine with A-form RNA structures of poly(rI)poly(rC) and poly(rA).poly(rU) has been studied by spectrophotometric, spectrofluorimetric, UV melting profiles, circular dichroism and viscometric analysis. The binding of sanguinarine to A-form duplex RNA structures is characterised by the typical bathochromic and hypochromic effects in the absorption spectrum, increasing stead...

In this study, derivative UV spectrophotometric methods were developed for the analysis of diflunisal, which is used as a nonsteroidal anti-inflammatory drug. Analysis wavelengths of diflunisal for the first and second order derivative UV spectrophotometric methods were obtained as 235.6 nm and 228.8 nm, respectively. The linearity ranges were found as 1.0-25.0 μg mL-1 for the first order and 1...