BACKGROUND Protective acquired immunity against helminths and allergic sensitisation are both characterised by high IgE antibody levels. Levels of IgE antibodies are naturally tightly regulated by several mechanisms including binding of the CD23 receptor. Following observations that helminth infections and allergic sensitisation may co-present, the current study aims to investigate the relation...

OBJECTIVE To evaluate the results of the tests used to identify the IgE mediated sensitization to Aspergillus fumigatus in patients with cystic fibrosis. METHODS This is a cross-sectional descriptive study with a convenience sample of 86 patients diagnosed with cystic fibrosis in the Reference Service in Cystic Fibrosis at a tertiary teaching hospital. The following tests were performed to as...

Background: Food hypersensitivity to walnut usually results in mild symptoms; however, several cases of anaphylactic reactions to this product have been observed. This study aimed to determine the immunochemical characteristics of the Persian walnut and provide the recombinant form of its main allergen. Materials and Methods: The allergenic proteins of th...

Mycoplasma pneumoniae is strongly associated with new onset asthma and asthma exacerbations. Until recently, the molecular mechanisms utilized by M. pneumoniae to influence asthma symptoms were unknown. However, we recently reported that an ADP-ribosylating and vacuolating toxin called the Community Acquired Respiratory Distress Syndrome toxin, CARDS toxin, produced by M. pneumoniae was suffici...

BACKGROUND In vitro testing is commonly used to diagnose and manage allergies. Clinical reactivity has been correlated with food-specific IgE levels by using the ImmunoCAP (Phadia, Uppsala, Sweden). OBJECTIVE To determine whether IgE levels derived from different assays are equivalent to those measured by ImmunoCAP. METHODS Fifty patients from the Mount Sinai Pediatric Allergy practice were...

The ability of substance P (SP) to regulate peak benzyl-penicilloyl (BPO)-specific IgE antibody-forming cell (AFC) responses in vivo and the ability of SP and other neuropeptides to regulate BPO-specific memory IgE AFC responses induced in vitro was determined. SP injected subcutaneously into BPO-keyhole limpet hemocyanin (BPO-KLH)-sensitized mice at the time of peak IgE responses suppressed th...

Methods We examined 50 formulae fed infants 1-12 months old with food allergy. The examination included a collection of allergy history, clinical evaluation and specific methods – the measurement of the coprofiltrate levels of total IgE and allergen-specific IgE to the cow milk protein and casein. The level of total IgE, cow and goat milk proteins IgE allergen-specific antibodies in coprofiltra...

BACKGROUND IgG to galactose-α-1,3-galactose (α-gal) are highly abundant natural antibodies (Ab) in humans. α-Gal-specific IgE Ab cause a special form of meat allergy characterized by severe systemic reactions 3-7 h after consumption of red meat. We investigated 20 patients who experienced such reactions and characterized their α-gal-specific IgE and IgG responses in more detail. METHODS α-Gal...

OBJECTIVES/HYPOTHESIS To evaluate local production of staphylococcal superantigen (SAg)-specific IgE in adenoid tissue and to compare its prevalence with that in the tonsil and serum, as well as its relationship with markers of allergic inflammation within adenoid tissue. STUDY DESIGN Prospective randomized study. METHODS We recruited 18 atopic children who had rhinitis symptoms and were se...