Oxygen molecule modulates tumour response to radiotherapy. Higher radiation doses are required under hypoxic conditions to induce cell death. Hypoxia may inhibit the non-homologous end-joining DNA repair through down regulating Ku70/80 expression. Hypoxia induces drug resistance in clinical tumours, although the mechanism is not clearly elucidated. Vaults are ribonucleoprotein particles with a ...

This paper presents a parametric stability study of groin, or cross vaults, structural element widely used in old masonry construction, particularly Gothic architecture. The vaults’ is measured using the geometric safety factor (GSF), computed by evaluating structure’s minimum thickness through thrust network analysis (TNA). obtained formulating and solving specific constrained nonlinear optimi...

Abstract De novo protein design is advancing rapidly. However, most designs are for single states. Here we report a de designed peptide that forms multiple ?-helical-bundle states accessible and interconvertible under the same conditions. Usually in such amphipathic ? helices associate to form compact structures with consolidated hydrophobic cores. recent rational computational have delivered o...

The molecular architecture of the rabbit skeletal muscle aldolase (D-fructose-1,6-bisphosphate D-glyceraldehyde-3-phosphate-lyase, EC 4.1.2.13) tetramer has been determined to 2.7-A resolution. Solution of the three-dimensional structure of rabbit muscle aldolase utilized phase information from a single isomorphous Pt(CN)4(2-) derivative, which was combined with iterative-phase refinement based...

0022-2836/$ see front matter © 2007 E Spontaneous membrane insertion and folding of β-barrel membrane proteins from an unfolded state into lipid bilayers has been shown previously only for few outermembrane proteins of Gram-negative bacteria. Here we investigated membrane insertion and folding of a human membrane protein, the isoform 1 of the voltage-dependent anion-selective channel (hVDAC1) o...

In this short paper we outline the data vault, a databaseattached external file repository. It provides a true symbiosis between a DBMS and existing file-based repositories. Data is kept in its original format while scalable processing functionality is provided through the DBMS facilities. In particular, it provides transparent access to all data kept in the repository through an (array-based) ...

arches and vaults are of the most important parts of traditional buildings of iranian architecture. in the traditional architecture of iran, before oil was discovered, fuel (such as wood) was one of the high-ranking issues. therefore, iranian traditional architects did their best to reduce the use of wood. there was however, a revival of the use of brick in construction. qomi-poosh technology i...

The outer membrane of Gram-negative bacteria is replete with a host of β-barrel outer membrane proteins (OMPs). Despite serving a variety of essential functions, including immune response evasion, the exact mechanism of OMP folding and membrane insertion remains largely unclear. The β-barrel assembly machinery complex is required for OMP biogenesis. Crystal structures and molecular dynamics (MD...

Most proteins adopt an approximate structural symmetry. However, they have no symmetry detectable in their sequences and it is unclear for most of these proteins whether their structural symmetry originates from duplication. As one of the six popular folds (super-folds) possessing an approximate structural symmetry, the triosephosphate isomerase barrel (TIM-barrel) domain has been widely studie...

BamA is the main component of the β-barrel assembly machinery (BAM) that folds and inserts outer membrane proteins in Gram-negative bacteria. Crystal structures have suggested that this process involves conformational changes in the transmembrane β-barrel of BamA that allow for lateral opening, as well as large overall rearrangements of its periplasmic POTRA domains. Here, we identify local dyn...