Glucokinase translocates between the cytoplasm and nucleus of hepatocytes where it is bound to a 68 kDa protein. The mechanism by which glucose induces translocation of glucokinase from the nucleus was investigated using glucose analogues that are not phosphorylated by glucokinase. There was strong synergism on glucokinase translocation between effects of glucose analogues (glucosamine, 5-thiog...

The primary purpose of this study was to process partially hydrolyzed PVOH grades (degree of hydroxylation (DH): 33-88%) via HME and to evaluate them as carrier for oral immediate release dosage forms in order to improve the release rate of poorly water soluble drugs (i.e., HCT and CEL) via the formulation of solid dispersions. PVOH grades (DH >70%) were able to solubilize HCT and CEL up to 15%...

the aim of the study was to evaluate changes of sorbitol, glucose, fructose, sucrose, and total sugars of plum fruits in different years under different climatic conditions i.e. air temperature and precipitation. the cultivars investigated in the research were: topstar, toptaste, jojo, haganta, tophit, and top 2000. the fruits were harvested at optimal stage of maturity in the experimental orch...

Actively growing Escherichia coli cells respond to increased osmotic pressure by increasing their internal pools of K+ (Epstein & Schultz, 1965) and of the amino acids glutamate, proline and a-aminobutyric acid (Britten & McClure, 1962; Measures, 1975). The change in amino acid pools may be brought about either by increased rates of their synthesis or by stimulation of the rate of their uptake....

Objetivou-se comparar a eficiência entre sete protocolos adaptados para extração de DNA genômico da espécie Prosopis juliflora (Sw). DC. Os utilizados tiveram como base em seus tampões: SDS 10%; CTAB 5%; Sorbitol, 3% e Sorbitol; 0,7% NaCl; 2%; 2% NaCl 1,4M. Em todos os testes, eliminou-se o uso β-mercaptoetanol, nitrogênio líquido, proteinase K RNAses. As amostras consistiram primórdios folhiar...

Faeces (n = 1319) were examined over three months for the presence of non-sorbitol fermenting, verotoxin producing Escherichia coli (serotype 0157). Seven isolates were found, four from patients with bloodstained diarrhoea and three from patients with no evidence of blood in the faeces. Screening of all faecal samples with specific 0157 antiserum for non-sorbitol fermenting organisms and agglut...

More than 90% of the strains of Escherichia coli O157:H7 that were identified on a MicroScan gram-negative dried conventional (overnight) panel gave one of two unique biochemical profile numbers that were not detected in other d-sorbitol-negative E. coli or in other strains isolated from pathogenic processes. This suggests that the panel has the capability of being used as a preliminary screeni...