نتایج جستجو برای: somaclonal variation
تعداد نتایج: 296663 فیلتر نتایج به سال:
Cell cultures of Mandevilla velutina have proved to be an interesting production system for biomass and secondary metabolites able to inhibit the hypotensive activity of bradykinin, a nonapeptide generated in plasma during tissue trauma. The crude ethyl acetate extract of cultured cells contains about 31- to 79-fold more potent anti-bradykinin compounds (e.g., velutinol A) than that obtained wi...
Soil salinization causes negative effects on plant productivity and poses an increasingly serious threat to the sustainability of agriculture. Improving salt and drought tolerance is one of the most diffi cult tasks for cereal breeders. A breeding program using somaclonal variation through biotechnological techniques has been used in rice to improve drought and salinity tolerance for increasing...
A simple, quick and efficient method for isolating total RNA from heavy browning cells was developed by adding polyvinylpyrrolidone, mercaptoethanol and 3 M NaAc during the process of the Trizol (a kind of a widely used RNA extraction buffer) method. High-quality total RNA was isolated and synthesized to cDNA. Transcript levels of four paclitaxel biosynthetic pathway genes: dxr, hmgr, ggpps and...
The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male fl owers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic em...
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