The DNA of Serratia marcescens has N6-adenine methylation in GATC sequences. Among 2-aminopurine-sensitive mutants isolated from S. marcescens Sr41, one was identified which lacked GATC methylation. The mutant showed up to 30-fold increased spontaneous mutability and enhanced mutability after treatment with 2-aminopurine, ethyl methanesulfonate, or UV light. The gene (dam) coding for the adenin...

The objective of this study was to evaluate the opinions and attitudes of dentists toward the use of rubber dam during various restorative procedures in their dental practices. A structured questionnaire was designed, and distributed to 300 dentists and those that were fully completed were collected for analysis. The collected informations about the opinions and attitudes of dentists toward the...

PURPOSE To investigate the relationship of age-related maculopathy, cataract, and glaucoma to visual acuity in the population-based Beaver Dam Eye Study. METHODS A cross-sectional, population-based study was performed in people 43 through 86 years of age residing in Beaver Dam, Wisconsin, who were identified between 1987 and 1988 and examined (n = 4926) between 1988 and 1990. Of those who par...

BACKGROUND Dams are important to ensure food security and promote economic development in sub-Saharan Africa. However, a poor understanding of the negative public health consequences from issues such as malaria could affect their intended advantages. This study aims to compare the malaria situation across elevation and proximity to dams. Such information may contribute to better understand how ...

Slit cleavage into N-terminal and C-terminal polypeptides is essential for restricting the range of Slit activity. Although the Slit cleavage site has been characterized previously and is evolutionally conserved, the identity of the protease that cleaves Slit remains elusive. Our previous analysis indicated that Slit cleavage is essential to immobilize the active Slit-N at the tendon cell surfa...

Microsoft SharePoint as a Digital Asset Management (DAM) System Harry Hurt Graphic Communication Department, June, 2010 Advisor: Dr. Harvey Levenson The purpose of this study is to determine to what degree Microsoft SharePoint can be used as a digital asset management (DAM) system. DAM systems are utilized to manage the creation, storage, utilization, and distribution of digital rich media file...

DNA adenine methylase (Dam(-)) mutants of Salmonella enterica serovar Typhimurium contain reduced levels of FinP RNA encoded on the virulence plasmid. Dam methylation appears to regulate finP transcription, rather than FinP RNA stability or turnover. The finP promoter includes canonical -10 and -35 modules and depends on the sigma(70) factor. Regulation of finP transcription by Dam methylation ...

Dam, the most described bacterial DNA-methyltransferase, is widespread in gamma-proteobacteria. Dam DNA methylation can play a role in various genes expression and is involved in pathogenicity of several bacterial species. The purpose of this study was to determine the role played by the dam ortholog identified in the entomopathogenic bacterium Photorhabdus luminescens. Complementation assays o...