BACKGROUND The human NOTCH4 gene is a candidate gene for schizophrenia due to its chromosomal location and neurobiological roles. In a British linkage study, NOTCH4 gene polymorphisms were highly associated with schizophrenia. In a Japanese case-control association study, however, these polymorphisms did not show significant associations with schizophrenia. We conducted a case-control study wit...

2.Merril, C.R., D. Goldman, S.A. Sedman and M.H. Ebert. 1981. Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science 211:1437-1438. 3.Sheffield, V.C., J.S. Beck, A.E. Kwitek, D.W. Sandstrom and E.M. Stone. 1993. The sensitivity of single-strand conformation polymorphism analysis for the detection of single base substitutions. G...

in this study, we report a mutation in cybb gene in a patient with x-cgd (diagnosed on the base of family history, ndt test, dhr 123 assay). mutation in cybb gene was detected using sscp analysis (single-strand conformation polymorphism) followed by sequencing. during screening for mutations in the cybb gene we observed 880 c t in exon 8. this mutation resulted in 290 arg stop. we also observed...

Patients with chronic hepatitis C are more likely to have significant changes in their physical and mental well-being than patients with liver disease of other etiology, and hepatitis C virus (HCV) has been occasionally implicated in diseases of the central nervous system. We analyzed the presence of the HCV negative-strand RNA sequence, which is the viral replicative intermediary, in autopsy b...

the sscp technique is based on the appearance of new “refolding” conformations during electro-phoresis due to mutation. in order to develop a simple, non-radioactive sscp analysis method so that it can reliably detect single nucleotide changes in pcr products up to 500 bp in length, extensive optimisation trials were performed . the best separation of sscp bands of pcr products up to 500 bp in ...

Placental Lactogen is a polypeptide hormone that is produced by the Placenta, also known as chorionic somatomammotropin hormone. It has both Growth Hormone and Prolactin activities on growth, lactation, and luteal steroid production. The objective of this study was to investigate the bovine Placental Lactogen (bPL) gene polymorphism of Holstein cows in Razavi Khorasan province. Blood samples we...

calpastatin (cast) is a specific inhibiter of calpains, playing a role in meat tenderization and myogenesis. in the present study, the polymorphism of the cast gene of makoei sheep was investigated by polymerase chain reaction and single strand conformation polymorphism technique (pcr–sscp). genomic dna was extracted from whole blood samples collected from 100 sheep. a 622 bp cast exon 1 segmen...

Placental Lactogen is a polypeptide hormone that is produced by the Placenta, also known as chorionic somatomammotropin hormone. It has both Growth Hormone and Prolactin activities on growth, lactation, and luteal steroid production. The objective of this study was to investigate the bovine Placental Lactogen (bPL) gene polymorphism of Holstein cows in Razavi Khorasan province. Blood samples we...