In fission yeast, RNA interference (RNAi)-dependent heterochromatin formation silences transgenes inserted at centromeres. In this issue, Bühler et al. (2007) demonstrate that the RNAi machinery directly targets transgene transcripts. Furthermore, they link transgene silencing to a protein complex resembling the TRAMP complex of budding yeast, which promotes transcript degradation via the exoso...

Metabolic exchange between an organism and the environment, including interactions with neighboring organisms, is important for processes of organismal development. Here we develop and use thin-layer agar natural product MALDI-TOF imaging mass spectrometry of intact bacterial colonies grown on top of the MALDI target plate to study an interaction between two species of bacteria and provide dire...

Asterixis was studied in nine patients, using a new electrophysiological technique: silent period locked averaging (SPLA). There were two types of electromyographic (EMG) silence in the movements clinically judged as asterixis. The jerky movement in one of the two types might be caused by the silent period after the subclinical cortical myoclonus. SPLA would be useful for studying asterixis as ...

Abstract In this paper, we propose a multi direction color interpolation method for multiple channel images. The proposed gradient judgment method silences the mediocre color interpolation artifacts. We adopted various weights from different directional gradients for conducting adaptive directional interpolation. The proposed weights are computed using correlation and intensity differences. The...

Silence infuses all aspects of our daily communications: what we say and remains unspoken; those to whom speak, ignore; trust respect, do not. In the school environment, interactions between students teachers lie at heart pedagogy. This relationship is also marked by its silences, concept ‘student voice’ arose out these silences on matters that affect students. However, role silence in such has...

‘O. what a world of unseen visions and heard silences, this insubstantial country of the mind! What ineffable essences, these touchless rememberings and unshowable reveries! And the privacy of it all! A secret theatre of speechless monologue and prevenient counsel, an invisible mansion of all moods, musings, and mysteries, an infinite resort of disappointments and discoveries. A whole kingdom w...

Silence can be harmful to patients, their loved ones, and doctors within the contexts of illness and bereavement. I draw from my experience with my son's illness and death to discuss five forms of silence--the silence around the experience of critical illness; the silence between life and death; the silence of doctors; the silence of the dead, and the silence of the ill--and of speech that may ...

The nervous system-enriched microRNA miR-124 is necessary for proper nervous system development, although the mechanism remains poorly understood. Here, through a comprehensive analysis of miR-124 and its gene targets, we demonstrate that, in the chordate ascidian Ciona intestinalis, miR-124 plays an extensive role in promoting nervous system development. We discovered that feedback interaction...

The global transcriptional regulator H-NS selectively silences bacterial genes associated with pathogenicity and responses to environmental insults. Although there is ample evidence that H-NS binds preferentially to DNA containing curved regions, we show here that a major basis for this selectivity is the presence of a conserved sequence motif in H-NS target transcriptons. We further show that ...