Two factors that interact specifically with the chromosomal replicator, autonomously replicating sequence 1 (ARS1), were identified in whole-cell yeast extracts. One of these proteins, ARS binding factor I (ABF-I), was purified to homogeneity as a polypeptide of 135 kDa. ABF-I binds within a region previously shown to be essential for the function of ARS1 when yeast are grown under certain nutr...

The H19 gene is subject to genomic imprinting because it is methylated and repressed after paternal inheritance and is unmethylated and expressed after maternal inheritance. We recently identified a 1.1-kb control element in the upstream region of the H19 gene that functions as a cis-acting silencer element in Drosophila. Here we investigate the function of this element in mice. We demonstrate ...

To minimize unwanted noise, the exhaust gas of high pressure and high temperature coming out of internal combustion engine passes through silencer. Various types of silencers like absorptive, reactive and resonating, perforated type are widely implemented in actual practice .perforated reactive mufflers have an effective damping capability. This paper deals with comparative structural analysis ...

The imprinting of the genes on human chromosome 11p15.5 is thought to be controlled by two imprinting control regions located in two differentially methylated CpG islands upstream of the H19 gene (H19 DMR) and in intron 10 of the KCNQ1 gene (KvDMR). We have examined sequences in the human 11p15.5 genomic imprinted region for the presence of insulators and silencers using a position- and enhance...

Engine noise is mainly from exhaust noise. Without adding muffler, at a certain speed, the noise up to 100 decibels. Reduce engine exhaust noise, and to high-temperature exhaust gas can be safely and effectively. Muffler as part of exhaust pipe, should ensure that the exhaust flow, resistance and sufficient strength. This research takes an exhaust silencer as a study object, and a muffler sound...

We engage the experimental and computational challenges of de novo regulatory module discovery in a complex and largely unstudied metazoan genome. Our analysis is based on the comprehensive characterization of regulatory elements of 20 muscle genes in the chordate, Ciona savignyi. Three independent types of data we generate contribute to the characterization of a muscle-specific regulatory modu...