The aim of the present investigation was to standardize in vitro shoot tip culture technique considering various aspects for direct plant formation two onion (Allium cepa L.) varieties ‘Indian’ and ‘Taherpuri’. About 1.5-2.2 mm tips were collected from bulbs sterilized with 0.1% HgCl2 duration. Then they cultured on MS medium supplemented hormonal concentrations different media composition prim...

BACKGROUND Aloe vera L. is an important pharmaceutical plant from which several medicinal and cosmetic compounds are extracted. Aloe is naturally propagated through offset, which is a slow and expensive labor cost method with low economical income. OBJECTIVES In this study, the effect of different media on shoot proliferation of the shoot tip of Aloe vera L. was investigated. MATERIALS AND ...

Shoot tips from actively growing, greenhouse maintained plants of Kalanchoe blossfeldiana were cultured in vitro for shoot proliferation and root initiation on Murashige and Skoog (MS) basal medium supplemented with NAA and BA, both in concentrations of 0.00, 0.50, 1.00 and 2.00 mg l-1. Results showed that the maximum plantlets height (7.012 cm), node number (4.516), root number (8.860) and roo...

Root and tuber crops (RTCs) are the second-most important carbohydrate commodity after cereals. Many species of RTCs vegetatively propagated, making their shoot tips preferred material to be conserved for future uses. Shoot tip cryopreservation provides an tool support long-term conservation plant genetic resources. Over past four decades, significant efforts have been undertaken move from rese...