Vaccinium myrtillus Linn. is an imported fruit tree with high economic value. Currently, in Vietnam there has not been any publication on vitro propagation of the blueberry plant. This study focused V. by nodal culture. The results showed that shoot growth and proliferation were best when nodes cultured WPM medium supplemented 0.5 mg/L zeatin, 30 g/L sucrose, 10 agar, pH 5.8 (shoot height 4.24 ...

abstract lettuce (lactuca sativa l.) is a major leafy vegetable and belongs to the asteraceae family (compositae). the genetic manipulation of lettuce requires a reliable and efficient regeneration method. since lettuce is a suitable bioreactor for production of recombinant proteins especially edible vaccines. this study was conducted to identify an appropriate method for lettuce gene transform...

parsi zira, bunium persicum (boiss.) b. fedtsch., or black zira, a member of the umbellifera family, is one of the most important medicinal species with high economic value. generally, there is limited information about in vitro culture of bunium persicum. in this study, the effect of various media (ms, b5) and combinations of plant growth regulators, naa and 2,4-d alone or together with kin, o...

The promotive effect of AgNO3 and aminoethoxyvinylglycine (AVG) on in vitro shoot regeneration from cotyledons of Brasska campestris ssp. peklnensls in relation to endogenous 1-aminocyclopropane-1-carboxylic acid (ACC) synthase, ACC, and ethylene production was investigated. AgNO3 enhanced ACC synthase activity and ACC accumulation, which reached a maximum after 3 to 7 days of culture. ACC accu...

In the present study, small segments of cotyledon, apical bud, hypocotyl, and root of 10-day-old seedlings were isolated and transferred to Murashige and Skoog (MS) base medium with different treatments of phytohormones. Two media were used for apical bud culture: MS with indole-3-acetic acid (IAA) and kinetin (Kin) (1 mg/l) and the other medium, MS with double vitamin of MS and 6-benzylaminopu...

In vitro multiple shoot regeneration of Solanum nigrum L., an Indian medicinal plant was accomplished on MS medium utilizing shoot tip and nodal explants. Direct multiple shoots differentiated within 6 weeks when explants were cultured on MS medium containing BAP (1.0-5.0 mg/l) and KIN (1.0-5.0 mg/l) individually. Among various concentrations of cytokinins tested, maximum number of multiple sho...

Cut-flower production of Rosa hybrida ‘Kardinal’ and ‘Fire and Ice’ were compared between two canopy management regimes: shoot-bending and hedgerow without shootbending, and between hydroponics and non-hydroponic (UC Mix) culture with a tensiometer-based irrigation system. We observed the number and length of all harvestable flowering shoots from September 1997 to August 1999 in order to assess...

Shoot-tip cultures of Pyrus elaeagrifolia Pallas, an important gene source for drought and chlorosis resistance in pear rootstock breeding, were established from a wild mature tree originated from seed. Murashige and Skoog basal medium supplemented with different concentrations of benzyladenine (BA) singly or in combination with auxin was used in the study. In the initial culture, the highest p...

A simple and efficient protocol for in vitro shoot and callus induction was established from three different explants of Canavalia gladiata (Jacq.) DC. an economically important legume. The explants viz., shoot buds, nodes, internodes were cultured in MS medium supplemented with various concentration and combination of (PGRs) Plant growth regulators 6-benzyl amino purine (BAP) (0.5mg/L – 1.5mg/...