The aim of the present study was to evaluate the acidification of the endosome-lysosome system of renal epithelial cells after endocytosis of two human immunoglobulin lambda light chains (Bence-Jones proteins, BJP) obtained from patients with multiple myeloma. Renal epithelial cell handling of two BJP (neutral and acidic BJP) was evaluated by rhodamine fluorescence. Renal cells (MDCK) were main...

Simple, accurate, precise and sensitive ultraviolet spectrophotometric and reversed-phase high-performance liquid chromatographic (RPHPLC) methods for simultaneous estimation of paracetamol (PCM) and nabumetone (NAB) in combined tablet dosage form have been developed and validated. Linearity of PCM and NAB was found in concentration range of 2-30 and 1-12 μg/mL in methanol at 249 and 230 nm, re...

Objective: In this present study, to analyze Gas Chromatography and Mass Spectrometry analysis of whole plant methanol extract of Adiantum capillus-veneris. Methods: Gas chromatography and mass spectrometry analysis of whole plant extract carried out with instrument GC-MS-QP 2010 [SHIMADZU]. Results: The methanol extracts of Adiantum capillus-veneris to identified thirty seven bioactive compoun...

Bacterial identification relies primarily on culture-based methodologies requiring 24 h for isolation and an additional 24 to 48 h for species identification. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is an emerging technology newly applied to the problem of bacterial species identification. We evaluated two MALDI-TOF MS systems with 720 consecu...

AIM A simple, accurate, precise, and reproducible reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the determination of rizatriptan benzoate in oral strip formulations. METHODOLOGY Separation was achieved under optimized chromatographic condition on a Hiper C18 column (250 mm × 4.6 mm, 5 m) using Shimadzu HPLC. The mobile phase consisted o...

This study evaluated the surface microhardness and fluoride release of 5 restorative materials - Ketac-Fil Plus, Vitremer, Fuji II LC, Freedom and Fluorofil - in two storage media: distilled/deionized water and a pH-cycling (pH 4.6). Twelve specimens of each material, were fabricated and the initial surface microhardness (ISM) was determined in a Shimadzu HMV-2000 microhardness tester (static l...

OBJECTIVES To evaluate fatigue of stud (ERA Overdenture (orange and white), Locator Root (pink) and OP anchor # 4) and magnetic (Magfit EX600W) attachments by measuring maximum retentive force. To compare retentive force of overdenture attachments after their reach stable retention. To determine minimum number of cycles required to reach stable retention. MATERIAL AND METHODS Three specimens ...

1H and 13C NMR spectra were recorded on a Bruker Avance III-500 or III-600 NMR spectrometer (chemical shifts with respect to (TMS) = 0 ppm). Solution electronic absorption and emission spectra were recorded on an Agilent 8453 spectrophotometer and Shimadzu 5301PC spectrofluorophotometer, respectively; a Perkin Elmer Spectrum Two UATR instrument was used to record FT-IR spectra of solid samples...

A stability-indicating high-performance liquid chromatographic method was developed and validated for the determination of Letrozole in tablet dosage forms. Reversed-phase chromatography was performed on Shimadzu Model LC-Class-Vp with Lichrocart/Lichrosphere 100 C-18 (250 mm×4.6 mm, 5 μm particle size) column with methanol: tetra butyl ammonium hydrogen sulfate (80:20V/V) as mobile phase at a ...