In the growing follicle, communication between the oocyte and its surrounding follicular cells is essential for normal oocyte and follicular development. Maturation of the fully grown oocyte in vivo is associated with the loss of cumulus cell-oocyte gap junctional communication, preventing entry of meiotic-modulating factors such as cAMP into the oocyte. We have previously shown that oocyte and...

OBJECTIVE To compare the outcomes of embryo development between oval-shaped oocyte as an abnormal morphology oocyte and a normal morphology oocyte in In Vitro Fertilization (IVF). METHODS This study was a comparative analytical study with retrospective approach which lasted from September 2014 until November 2015. For this study, we used secondary data (medical records) from 24 patients submi...

objective(s): several researchers have reported the relationship between infertility in male and varicocele for so many years but the implication of varicocele in female patients is remains elusive. here, we aim to examine the effects of unilateral varicose ovarian vein on antioxidant capacity and oocyte quality of rat ovary after the experimental creation of varicocele in female rats. material...

rate of in vitro maturation of oocytes is one of the challenges of assisted reproductive techniques. in this study we investigated the effects of supplementation of cysteamine on the rate of in vitro maturation of oocytes in two different media. germinal vesicle oocytes were collected from mouse ovary and cultured in two media (tcm199 and meme) with 0, 50, 100, 200, 500 µm/ml cysteamine. number...

the purpose of this study was to evaluate the use of glass capillary micropipette (gcm) as a vessel forvitrification of bovine oocytes. cumulus-oocyte complexes (cocs) were obtained from slaughter-house andwashed 5 to 6 times in the washing medium (tcm-199 + 20% fbs) and randomly assigned to treatment andcontrol group. in the first step of vitrification, cocs were exposed to first vitrification...

pelvic inflammatory disease (pid) is a rare complication of transvaginal oocyte retrieval. it may result in failure of assisted reproductive techniques (art). during a 7 years period, 5958 transvaginal ultrasound-guided oocyte retrievals resulted in 10 cases of acute pid. eight out of 10 patients were diagnosed infertile because of endometriosis. two patients had mild ovarian, 3 had stage iii, ...

objective: currently, there is no agreement on the optimal urinary derived human chorionic gonadotropin (u-hcg) dose requirement for initiating final oocyte maturation prior to oocyte collection in in vitro fertilization (ivf), but doses that range from 2500- 15000 iu have been used. we intended to determine whether low dose u-hcg was effective for oocyte maturation in ivf/intracytoplasmic sper...

Cell-to-cell communication was characterized in cumulus-oocyte complexes from rat ovarian follicles before and after ovulation. Numerous, small gap junctional contacts were present between cumulus cells and oocytes before ovulation. The gap junction are formed on the oocyte surface by cumulus cell processes that transverse the zona pellucida and contact the oolemma. The entire cumulus mass was ...

CAPSULE Mechanisms that control the survival of oocytes and, by extension, the duration of ovarian function have been identified. However, it is still not clear whether oocyte "quality" is related to survival, nor is the role of the granulosa cells of follicles in follicle survival entirely understood. Here, we consider oocyte-intrinsic and oocyte-extrinsic mechanisms of oocyte loss and argue t...