A tetracycline-regulated expression system is combined with the FLAG-epitope tagging method for conditional expression of potentially toxic proteins in mammalian cells. This strategy allows a controlled expression of exogenous gene products and also provides a unique way of protein purification. Two mammalian expression plasmids containing the FLAG sequence and flanking multiple cloning sites w...

WU polyomavirus was detected in nasopharyngeal aspirates in 2 (2.5%) of 79 children with respiratory infections (both infected with respiratory syncytial virus) and in 5 (6.4%) of 78 asymptomatic children during the same winter season in Canada. The strains were closely related to Australian and American viruses based on analysis of large T antigen (TAg) and VP2 genes. The pathogenic role of WU...

T he human brain is organized into a collection of interacting networks with specialized functions to support various cognitive functions. The word “connectome” first burst on the scene with the work of Sporns et al. [1], who urged brain researchers to advance a comprehensive structural description of the elements and connections forming the human brain. An increasing body of evidence indicates...

In 1991, Chang and Wu proposed a broadcasting cryptosystem using interpolating polynomials and geometric properties of circles. Nevertheless, the Chang-Wu scheme can be further enforced by using a time-variant parameter and a one-way function to protect legal receivers’ secrets from being disclosed. The authors present an improvement of the Chang-Wu scheme using geometric properties of lines. T...

BACKGROUND/AIMS Hyperuricemia is an independent risk factor for chronic kidney disease and cardiovascular disease. Here, we examined the combined protective effects of Chinese herbal formula Si-Wu-Tang and Er-Miao-San on hyperuricemia and renal impairment in rats. METHODS Rats were randomly divided into normal rats, hyperuricemic rats, and hyperuricemic rats orally administrated with benzbrom...