To evaluate a new method for measuring pancreatic amylase in serum, in which the salivary isoenzyme is inhibited with a specific monoclonal antibody, we determined the activity of pancreatic and salivary amylase in sera from 103 healthy subjects and from 114 hospitalized patients having a wide range of total amylase activities. CVs for the proposed method ranged from 0.8% to 5.1% (within day) a...

CONTEXT Salmonella has been identified as a causative agent of acute pancreatitis. OBJECTIVE We prospectively evaluated the frequency of acute pancreatitis, pancreatic enzyme elevation and morphological pancreatic abnormalities in patients with Salmonella infection. SUBJECTS Thirty consecutive patients with salmonellosis (Salmonella enterica serovar Enteritidis: n=25; Salmonella enterica se...

Elevation of serum amylase level is usually associated with pancreatitis, but 2% of cases are associated with medication. Only 1 case of pancreatitis caused by sertraline has been reported. Although it has been reported that selective serotonin reuptake inhibitors may rarely cause pancreatitis, Elevated serum amylase levels associated with sertraline use in cases without pancreatitis has not be...

BACKGROUND Alterations in serum biomarkers have been used to evaluate for pancreatitis in the emergency department (ED). Studies have shown lipase to be as sensitive and more specific than amylase in diagnosing pancreatitis and that amylase plus lipase does not improve accuracy over lipase alone. OBJECTIVE To determine effects of interventions to decrease ordering of amylase in the evaluation...

Amylases are an important family of enzymes involved in insect carbohydrate metabolism that are required for the survival of insect larvae. For this reason, enzymes from starch-dependent insects are targets for insecticidal control. PF2 (Olneya tesota) is a lectin that is toxic to Zabrotes subfasciatus (Coleoptera: Bruchidae) larvae. In this study, we evaluated recognition of the PF2 lectin to ...

The protein contents and binding modes of immunoglobulin-amylase complexes were investigated. Amylase staining after immunoelectrophoresis permitted identification of IgA-lambda type in three cases, IgA-kappa type in two cases and IgG-kappa type in one case. The precipitin line of Fab fragment of IgA in four patient's sera after papain digestion was found to have amylase activity. Sucrose densi...

Pancreatic juice and serum from patients with acute pancreatitis contain three enzymes that have lipolytic activity: L1 and L2, which are pancreatic isoenzymes or isoforms of lipase (EC 3.1.1.3), and L3, which is probably pancreatic carboxyl ester lipase, also known as cholesterol esterase (EC 3.1.1.13). These enzymes are readily separated electrophoretically on agarose and can be developed wit...

tient presented with malaise, sore throat, and cough with neither abdominal nor back pain at 36 weeks of gestation. She was in no acute distress. Her white blood cell count was 9.5 10/l, C-reactive protein 18 mg/l, plasma glucose 60 mg/dl, and serum amylase 683 IU/l. Ultrasonography yielded no medically or obstetrically abnormal findings. Lack of signs of acute pancreatitis suggested acute tons...

The presence of ar-amylase in liver tissue has been reported previously by several investigators (l-3). In the liver from rats deprived of food, the enzyme is found primarily in the microsomal fraction (4) whereas in fed animals a considerable proportion is bound to glycogen (5). In perfused liver systems, amylase activity is secreted into the perfusion medium under conditions in which other en...

Diagnosis of acute pancreatitis (AP) is erroneous in up to one third of patients when based on clinical criteria and elevated serum amylase values. Furthermore, according to autopsy reports fatal pancreatitis remains clinically undiagnosed in 22 to 86 % of hospitalised patients. Consequently, search for better methods for the diagnosis of AP seems not only justified but urgent. The pancreas sec...