Lumpy skin disease (LSD) is a notifiable, transboundary cattle that spreads rapidly and has relevant economic impact. The etiological agent the LSD virus (LSDV), genus Capripoxvirus, family Poxviridae. To date, LSDV widely present in Africa, Asia, transcontinental regions like Russia, Turkey, Middle East, thus representing continuous threat to free neighbours. Appropriate serosurveillance progr...

Structural human leukocyte antigen (HLA) matching at the eplet level can be identified by HLAMatchmaker, which requires the entry of four-digit alleles. The aim of this study was to evaluate the agreement between eplet mismatches calculated by serological and two-digit typing methods compared to high-resolution four-digit typing. In a cohort of 264 donor/recipient pairs, the evaluation of measu...

Autoantibodies can be detected in autoimmune diseases with a long prodromal phase and may serve as early indicators of disease activity. Autoantibody-based screening methods are therefore potent tools for the identification of target antigens. The SEREX method (serological identification of antigens by recombinant expression cloning) has been developed for the serological definition of immunoge...

BACKGROUND Serological methods to monitor Pseudomonas aeruginosa colonisation in patients with cystic fibrosis (CF) are advocated but the diagnostic value of a commercially available P aeruginosa antibody test to detect early and chronic P aeruginosa colonisation in a non-research setting has not been assessed. METHODS Colonisation with P aeruginosa was estimated by regular culture of sputum ...

Methods: Seventeen groups of 30 to 60 sows and gilts in seven herds were monitored over a 2-year period by serological testing for the pathogens listed above. Litter size, number of stillborn (including mummies), average weaning weight, preweaning deaths, and interfarrowing interval were recorded for the sows that were tested. Multiple linear regression analyses were performed to evaluate the a...

Brucellosis is an urgent infectious disease of livestock and wild animals and the commonest human zoonosis. Diagnosis of brucellosis is rather complicated and it has to be obligatorily confirmed by laboratory testing. Direct bacteriological and molecular methods and indirect serological tests are used for brucellosis diagnostics. The choice of the diagnostic tools depends on the overall epidemi...

8. Kantsø B, Svendsen CB, Jørgensen CS, Krogfelt KA. Evaluation of serological tests for the diagnosis of rickettsiosis in Denmark. J Microbiol Methods. 2009;76:285–8. doi:10.1016/j. mimet.2008.12.012 9. Halle S, Dasch G. Use of a sensitive microplate enzyme-linked immuno-sorbent assay in a retrospective serological analysis of a laboratory population at risk to infection with typhus group rick...

background and aims: celery mosaic virus (cemv) is one of the causal agents of viral diseases in celery (apium graveolens). cemv is a member of the potyvirus genus in potyviridae family. the virus is naturally transmitted by aphids in a non-persistent manner. during growing season 2006-2007 viral disease symptoms were observed in celery fields grown in tehran province (bage daneshkade, mohamads...

alfalfa crops were surveyed for the incidence of alfalfa mosaic virus (amv), cucumber mosaic virus (cmv), peanut stunt virus (psv), bean leaf roll virus (blrv), bean yellow mosaic virus (bymv) and bean common mosaic virus (bcmv) in the major growing areas in the southeast and central regions of iran. samples were collected between may 2009 and march 2011 and analyzed for viral infection initial...