Abstract Serodiagnosis of SARS-CoV-2 infection is impeded by immunological cross-reactivity among the human coronaviruses (HCoVs): SARS-CoV-2, SARS-CoV-1, MERS-CoV, OC43, 229E, HKU1, and NL63. Here we report identification humoral immune responses to peptides that may enable discrimination between exposure other HCoVs. We used a high-density peptide microarray plasma samples collected at two ti...

The indirect fluorescent antibody test was used to detect antibodies to Trichomonas vaginalis in 200 antenatal patients. A total of 104 (52%) gave a positive reaction with antigen prepared from cultures of trichomonas isolated from seven of the patients. Antitrichomonal antibody was detected at a 1/4 dilution in 90% of patients with proven trichomoniasis, while the highest dilution at which ant...

BACKGROUND A new human-pathogenic parvovirus, human bocavirus (HBoV), has recently been discovered and associated with respiratory disease in small children. However, many patients have presented with low viral DNA loads, suggesting HBoV persistence and rendering polymerase chain reaction-based diagnosis problematic. Moreover, nothing is known of HBoV immunity. We examined HBoV-specific systemi...

Various serological techniques including immunoprecipitation, indirect haemagglutination and the indirect fluorescence antibody test have been successfully applied for the detection of fasciolosis, mainly in experimental infections. Recently, the most frequently used assay is the ELISA technique with somatic or excretory-secretory antigens obtained from adult flukes. Further purification of suc...